Pathological and immunohistochemical evaluation of wound healing potential of Nigerian bee propolis in albino rats

This study evaluated the efficacy of the Nigerian bee propolis as a wound healing agent, on full thickness skin wounds of healthy adult male (150.0  ± 0.5 g) albino rats randomly divided into three treated groups (propolis extract (PE), propylene glycol (PG), silver sulfadiazine) and an untreated group. Each rat had three circular full thickness skin wounds created on the cranial, middle, and caudal surface of the rat’s dorsum. The woun ds in each group were topically treated with bee propolis extract (PE, 0.1 ml), propylene glycol (PG, 0.1 ml) and silver sulfadiazine (SS, 0.1 ml) twice daily for 21 days, except the untreated group (UT). The wounds were evaluated for gross (exudation, edema, hyperemia, wound contraction), histo logic (granulation, angiogenesis, fibroplasia, epithelialization), and expression of epidermal growth factor (EGF) using standard techniques. Data was descriptively summarized as percentages, mean and analyzed using Chi-square and analysis of variance atα = 0.05. Wound edge edema (WEE), hyperemia, and exudation were prominent in all the groups between days 0–2. WEE was significantly less on day 3 in the PE (14%) and SS (14%) groups, compared with the PG (35%) and UT (66%) groups. Wounds treated with PG and UT were significantly more hyperemic t han those with PE and SS. Wound contraction was significantly less on day 2 in the UT wounds when compared to the treated wounds (PE = 12.63%, SS = 2.22%, PG = 4.94%, and UT = â...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research