MicroRNA-1204 promotes cell proliferation by regulating PITX1 in non-small cell lung cancer.

MicroRNA-1204 promotes cell proliferation by regulating PITX1 in non-small cell lung cancer. Cell Biol Int. 2018 Dec 14;: Authors: Jiang W, He Y, Shi Y, Guo Z, Yang S, Wei K, Pan C, Xia Y, Chen Y Abstract MicroRNA-1204 (miR-1204), a member of the PVT1 region, may improve B cell differentiation and metastasis in breast cancer. However, the role of miR-1204 in NSCLC (non-small cell lung cancer) and its mechanism remain unclear. The GEO public database was first employed to find differentially expressed genes. The expression level of miR-1204 in patient tissues and NSCLC cell lines were determined using qRT-PCR. Cell proliferation assays were performed to investigate the impact of miR-1204 on cell growth. Bioinformatics analysis and dual-luciferase reporter assays were conducted to find potential target genes. Finally, we performed in vivo experiments to identify the effect of miR-1204 on tumor formation in nude mice. It was first found that miR-1204 was overexpressed in NSCLC tissues and cells. miR-1204 increased the proliferation of NSCLC cells and reduced cell cycle arrest in vitro. PITX1 (paired like homeodomain 1) was found as a potential target gene. In addition, PITX1 was also found to be low in expression in NSCLC tissues and cells. To show that PITX1 reversed the function of miR-1204 in promoting proliferation, confirmatory experiments were performed. Moreover, high miR-1204 and low PITX1 expression were highly correlated with ...
Source: Cell Biology International - Category: Cytology Authors: Tags: Cell Biol Int Source Type: research