Duodental bypasses surgery improves type 2 diabetes in rat model through regulation of early glucose metabolism

This study aimed to measure the effects of duodenal-jejunal exclusion (DJE) on glycometabolism in non-obese type 2 diabetic rats, and to investigate the mechanisms. Goto–Kakizaki(GK) rats and Sprague–Dawley(SD) rats were divided into DJE operation groups and sham operation groups respectively. The glucose relative parameters were measured before and after operation. 8 weeks postoperation, the levels of the key regulators of intestinal gluconeogenesis and the crucial proteins of hepatic insulin signaling were evaluated. Postoperatively, the concentration of blood glucose declined and the insulin sensitivity was increased significantly in diabetic rats. However, there was no obvious reduction of the weight. 8 weeks postoperatively, the mRNA levels of glucose-6-phosphatase and phosphoenolpyruvate pyruvate kinase in jejunum and the levels of insulin receptor substrate-2 and glucose transporter-2 in liver were increased obviously compared with sham operation. DJE surgery is an effective procedure for improving glucose metabolism independent of weight loss in non-obese diabetic rats. The molecular mechanisms might be related to a series processes including intestinal gluconeogenesis and hepatic insulin signaling pathway.
Source: Canadian Journal of Diabetes - Category: Endocrinology Source Type: research