Expression of KLF9 in pancreatic cancer and its effects on the invasion, migration, apoptosis, cell cycle distribution, and proliferation of pancreatic cancer cell lines.

Expression of KLF9 in pancreatic cancer and its effects on the invasion, migration, apoptosis, cell cycle distribution, and proliferation of pancreatic cancer cell lines. Oncol Rep. 2018 Oct 02;: Authors: Zhong Z, Zhou F, Wang D, Wu M, Zhou W, Zou Y, Li J, Wu L, Yin X Abstract Kruppel-like factor 9 (KLF9), a transcription factor, is critical for the inhibition of growth and development of tumors, whereas its effects in pancreatic cancer remains unclear. The purpose of the present study was to investigate the expression and functional significance of KLF9 in vitro, by assessing the expression of KLF9 in pancreatic cancer tissue samples and its association with the total survival of patients and clinicopathological data. The levels of KLF9 expression in adjacent tissues and pancreatic cancer tissues were detected using immunohistochemistry. Using western blot analyses, we assessed KLF9 expression in human pancreatic cancer cell lines. Using flow cytometric analysis and CCK-8, we evaluated the effects of KLF9 expression on cell apoptosis, the cell cycle and proliferation of pancreatic cancer cells. Its effects on migration and cell invasion were detected by performing Transwell assay. By conducting western blot analyses, we evaluated the expression of relative target proteins (involved in invasion, migration, apoptosis, and cell cycle distribution. Our results revealed that in both tissue samples and cell lines (particularly in BxPC-3 ...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research