Pterostilbene protects against acetaminophen-induced liver injury by restoring impaired autophagic flux.

In this study, we investigated the protective mechanisms of PTE against APAP-induced liver injury, focusing on autophagy. ICR mice were intraperitoneally (i.p.) treated with 400 mg/kg of APAP. PTE (15, 30, and 60 mg/kg, i.p.) and chloroquine (CQ, 60 mg/kg, i.p.) were injected 1 h after APAP treatment. Blood and liver tissues were isolated 6 h after APAP treatment. PTE decreased serum aminotransferase activities and hepatic oxidative stress; this protective effect was abolished by CQ. APAP impaired autophagic flux, as evidenced by increased microtubule-associated protein-1 light chain 3-II and p62 protein expression; this impaired autophagic flux was restored by PTE, while CQ abolished this effect. APAP decreased beclin-1 and autophagy related protein 7 protein expressions, while PTE attenuated these decreases. PTE increased the lysosome-associated membrane protein-2 protein expression and decreased the mammalian target of rapamycin (mTOR) and Unc-51 like autophagy activating kinase 1 phosphorylation. Our findings suggest that PTE protects against APAP-induced hepatotoxicity by enhancing autophagic flux. PMID: 30543896 [PubMed - as supplied by publisher]
Source: Food and Chemical Toxicology - Category: Food Science Authors: Tags: Food Chem Toxicol Source Type: research