Di-2-ethylhexylphthalate promotes thyroid cell proliferation and DNA damage through activating thyrotropin-receptor-mediated pathways in vitro and in vivo.

Di-2-ethylhexylphthalate promotes thyroid cell proliferation and DNA damage through activating thyrotropin-receptor-mediated pathways in vitro and in vivo. Food Chem Toxicol. 2018 Dec 10;: Authors: Kim S, Park GY, Yoo YJ, Jeong JS, Nam KT, Jee SH, Lim KM, Lee YS Abstract Phthalates are being suggested to be associated with altered thyroid function and proliferative changes, but detailed mechanisms remain unclear. Here, we examined the effects of di-(2-ethylhexyl) phthalate (DEHP) on DNA damage and proliferation in thyroid using thyroid carcinoma cell line, 8505C, in vitro and the rats orally treated with DEHP at 0, 0.3, 3, 30 and 150 mg/kg for 90 days from post-natal day 9 in vivo. Exposure to DHEP (1-50 μM) induced cellular proliferation, as evidenced by increased cell viability and DNA synthesis. Activation of γH2AX, a sensitive biomarker for DNA damage was observed following the exposure to DHEP (from 5 to 50 μM) with increased comet tail moment (5-100 μM) in comet assay, reflecting that DNA damage also occurred. When upstream signaling was examined, both thyrotropin receptor (TSHR)-ERK1/2 axis and TSHR-AKT axis were activated with upregulation of Pax8, a master transcriptional factor for thyroid differentiation and proliferation. Thyroid tissue from juvenile rats orally exposed to DEHP also confirmed DNA damage responses and the activation of TSHR signaling, which was evident from 0.3 to 3 mg/kg respectively. Notably, deletio...
Source: Food and Chemical Toxicology - Category: Food Science Authors: Tags: Food Chem Toxicol Source Type: research

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ConclusionmSLNB showed satisfactory performance in PTC with clinical-negative nodes. We have shown mSLNB to be a good predictor of central compartment status that can improve the staging and management of PTC patients.
Source: European Journal of Surgical Oncology (EJSO) - Category: Surgery Source Type: research
In conclusion, the present study suggested that TGF-β1 may promote the invasion and migration of PTC cells by inhibiting the expression of lncRNA-NEF. PMID: 30867742 [PubMed]
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
Authors: Koshikawa T, Fujita N, Ueda N, Ota Y, Sasaki E, Murakami Y, Hosoda W, Yatabe Y, Hanai N, Higuchi M, Hirokawa M, Miyauchi A Abstract We studied cytological specimens of conventional papillary thyroid carcinoma (PTC), follicular variant papillary thyroid carcinoma (FVPTC), and noninvasive follicular thyroid tumor with papillary-like nuclear features (NIFTP) (formerly noninvasive FVPTC) to identify useful cytological parameters for their differentiation. Cytological findings of invasive FVPTC and NIFTP were very similar to each other but differed from those of conventional PTC. Intranuclear cytoplasmic inclus...
Source: Endocrine Journal - Category: Endocrinology Tags: Endocr J Source Type: research
Conclusions The results of the present study showed that vemurafenib increased the expression of anti-apoptotic proteins of the BCL-2 family. Thus, the combination of vemurafenib with navitoclax may be effective in BRAFV600E-positive PTC treatment. PMID: 30869573 [PubMed - as supplied by publisher]
Source: Thyroid : official journal of the American Thyroid Association - Category: Endocrinology Tags: Thyroid Source Type: research
CONCLUSION: MMR-D tumors comprised 14% of our ATC cohort. Although the findings must be interpreted with caution given the small number of MMR-D ATC in our cohort, our results suggest that MMR status may be prognostically significant in ATC. PMID: 30869569 [PubMed - as supplied by publisher]
Source: Thyroid : official journal of the American Thyroid Association - Category: Endocrinology Tags: Thyroid Source Type: research
AbstractBackgroundCentral lymph node metastasis (LNM) in papillary thyroid carcinoma (PTC) is common. But the association between primary tumor characteristics and specific features of metastatic lymph nodes in PTC has not been fully identified. Determining risk factors for LNM may help surgeons determine rational extent of lymph node dissection.MethodsData from 432 patients who underwent thyroidectomy with cervical lymph node dissection for PTC were retrospectively analyzed. The relationships between LNM to central compartment or lateral compartment and clinicopathologic factors were analyzed. Cox regression model was use...
Source: Clinical and Translational Oncology - Category: Cancer & Oncology Source Type: research
Metastatic follicular thyroid carcinoma (FTC) is rare. The aim of this study was to determine the clinical, histologic, and molecular differences between patients with metastatic FTC who present with distant metastatic (DM) disease versus those who present with a primary thyroid nodule (PT). Clinical and pathologic information was extracted from the medical record and surgical pathology report. When available, slides were reviewed. Molecular testing was performed on available primary and/or metastatic lesions. Thirty-six patients with metastatic FTC were identified: 15 DM and 21 PT. DM patients were significantly older tha...
Source: The American Journal of Surgical Pathology - Category: Pathology Tags: Original Articles Source Type: research
Authors: Ahn J, Song E, Oh HS, Song DE, Kim WG, Kim TY, Kim WB, Shong Y, Jeon M Abstract BACKGROUND: The lymphocyte-to-monocyte ratio (LMR), which reflects the tumor infiltrating immune cell status and host immunity, has been reported as a prognostic marker in various cancers. The aim of the present study was to evaluate the role of LMR as a prognostic marker in predicting the survival of patients with anaplastic thyroid carcinoma (ATC). METHODS: We retrospectively included 35 ATC patients with available complete blood cell count (CBC) data. The primary outcome was the overall survival (OS) of patients with ATC...
Source: Thyroid : official journal of the American Thyroid Association - Category: Endocrinology Tags: Thyroid Source Type: research
Authors: Oh HS, Kwon H, Song E, Jeon M, Kim TY, Lee JH, Kim WB, Shong Y, Chung KW, Baek JH, Kim WG Abstract Background Tumor volume (TV) of papillary thyroid carcinoma (PTC) increases exponentially during active surveillance and the growth rate differs for each patient. TV doubling time (TVDT) is considered as a strong dynamic marker for the prediction of growth rate and progression of the tumor. Methods In this cohort study, we analyzed 273 PTC patients who underwent active surveillance for over one year rather than immediate thyroid surgery. TVDT was calculated in each patient and patients were divided into two g...
Source: Thyroid : official journal of the American Thyroid Association - Category: Endocrinology Tags: Thyroid Source Type: research
AbstractMalignancy developing in thyroglossal cysts is very rare. Papillary carcinoma is the most common histopathological type of carcinoma encountered in thyroglossal cysts followed by squamous cell carcinoma. A 50-year-old male presented with a midline neck swelling. After ultrasonography and fine needle aspiration cytology, he underwent Sistrunk operation for removal of the thyroglossal cyst. The histopathology was reported as papillary carcinoma of the thyroid. So, he underwent total thyroidectomy, which showed foci of papillary microcarcinoma. Clinical awareness of this rare condition is essential for proper manageme...
Source: Indian Journal of Surgical Oncology - Category: Cancer & Oncology Source Type: research
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