1,25 ‑Dihydroxyvitamin D regulates macrophage polarization and ameliorates experimental inflammatory bowel disease by suppressing miR-125b.

This study aimed to explore whether 1,25(OH)2D3 modulates macrophage polarization in inflammation. Peripheral blood mononuclear cells and colitis mice were treated with 1,25(OH)2D3. Macrophages were transfected with siRNA-vitamin D receptor (VDR) or miR-125b mimic or inhibitor, and 1,25(OH)2D3-pretreated colitis mice were injected with a miR-125b agomir. The distribution of macrophage subsets and macrophage subtype characteristics was analyzed. As expected, 1,25(OH)2D3 transformed lipopolysaccharide-induced M1 macrophages to the M2 subset, downregulated tumor necrosis factor-α and interleukin (IL)-6 expression and interferon regulatory factor 5 (IRF5) phosphorylation, and upregulated IL-10, arginase-1, VDR, and IRF4 expression. SiRNA-VDR and miR-125b mimic significantly impaired 1,25(OH)2D3 activity. In colitis mice, 1,25(OH)2D3 pretreatment ameliorated disease activity, converted M1 macrophages to the M2 subtype, suppressed IRF5 phosphorylation, and increased IRF4 expression in lamina propria mononuclear cells (LPMC). miR-125b agomir injections reversed 1,25(OH)2D3 action. Collectively, the results demonstrate that 1,25(OH)2D3 downregulates miR-125b expression and promotes M1 macrophage polarization to the M2 subtype. 1,25(OH)2D3 pretreatment ameliorated colitis by restoring the LPMC macrophage subtype balance. PMID: 30540970 [PubMed - as supplied by publisher]
Source: International Immunopharmacology - Category: Allergy & Immunology Authors: Tags: Int Immunopharmacol Source Type: research

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en TN Abstract Vitamin D deficiency is an established risk factor for many autoimmune diseases and the anti-inflammatory properties of vitamin D underscore its potential therapeutic value for these diseases. However, results of vitamin D3 supplementation clinical trials have been varied. To understand the clinical heterogeneity, we reviewed the pre-clinical data on vitamin D activity in four common autoimmune diseases: multiple sclerosis (MS), rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), and inflammatory bowel disease (IBD), in which patients are commonly maintained on oral vitamin D3 supplementa...
Source: Journal of Autoimmunity - Category: Allergy & Immunology Authors: Tags: J Autoimmun Source Type: research
The Wheat Belly lifestyle begins with elimination of the worst and most dominant of all grains in the diet, semi-dwarf wheat products, followed by elimination of its closely-related brethren in other grains. This alone is a powerful start in reversing the 200-some diseases of autoimmunity. We now know that the gliadin protein of wheat and related proteins in other grains trigger increased intestinal permeability that initiates the process, as highly inflammatory compounds, such as lipopolysaccharide from bacterial cell walls, are permitted entry into the body. We also know that gliadin itself gains entry into the bloo...
Source: Wheat Belly Blog - Category: Cardiology Authors: Tags: News & Updates autoimmune Gliadin gluten-free grain-free grains Inflammation wheat belly Source Type: blogs
When I was a kid, grasshoppers were everywhere. I walked through a field every day to get to school and grasshoppers were everywhere, jumping back and forth across my path, frequently banging off my legs. At night in summer, the backyard was filled with fireflies that we’d chase and capture in jars to watch up close. And there were butterflies of many colors and varieties everywhere, flitting from flower to flower. Today, I don’t see any grasshoppers. In fact, I haven’t seen one in over 40 years. I saw one—just one—firefly this past summer in my backyard. And I can count the number of butterie...
Source: Wheat Belly Blog - Category: Cardiology Authors: Tags: Agribusiness bowel flora Inflammation microbiota prebiotic probiotic wheat belly Source Type: blogs
This study aimed to perform a systematic review and meta-analysis on the association between vitamin D levels and AA.
Source: Journal of the American Academy of Dermatology - Category: Dermatology Authors: Tags: Research letter Source Type: research
CONCLUSION: The literature does not show significant differences between the PS and PsA groups in terms of serum 25-OHD levels and a prevalence of Vitamin D deficiency. Besides, PASI scores were higher in the PS group. CRP values in the PsA group were higher than in the PS group. There was a poor negative correlation between CRP and serum 25-OHD levels in the PsA group. This correlation was not found in the PS group. PMID: 28946518 [PubMed - as supplied by publisher]
Source: Journal of Back and Musculoskeletal Rehabilitation - Category: Orthopaedics Tags: J Back Musculoskelet Rehabil Source Type: research
Abstract Vitamin D deficiency and insufficiency are associated with an increased risk of cancer, autoimmune disease, inflammation, infection, cardiovascular disease and metabolic disease, as well as bone and mineral disorders. The vitamin D receptor (VDR), a member of the nuclear receptor superfamily, is a receptor for the active form of vitamin D, 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3], and mediates vitamin D regulation of specific target gene expression. The secondary bile acid lithocholic acid, which is produced by intestinal bacteria, is another natural VDR ligand. VDR signaling has been suggested to be...
Source: Current Medicinal Chemistry - Category: Chemistry Authors: Tags: Curr Med Chem Source Type: research
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Source: Current Gastroenterology Reports - Category: Gastroenterology Source Type: research
The advice you’re getting from your doctor, the TV and even the Surgeon General is so wrong, it’s scary. They’re all busy telling you to stay out of the sun. But Swedish researchers recently discovered that nonsmokers who avoid the sun have a life expectancy similar to smokers who spend a lot of time in the sun. Staying out of the sun is as dangerous as smoking.1 It doesn’t surprise me. I tell all my patients sunlight exposure is essential to our health because it is the best source of vitamin D… possibly the most important nutrient we know of. Mainstream medicine still doesn’t recogni...
Source: Al Sears, MD Natural Remedies - Category: Complementary Medicine Authors: Tags: Health Source Type: news
Conclusions: Our study highlights the association between vitamin D deficiency and pretreatment positivity for ANA with the risk for anti-TNF failure and adverse events, and the inverse relationship between vitamin D levels and ANA. Due to the high prevalence of vitamin D deficiency in IBD and the immune-mediated nature of the disease, these elements should be evaluated before starting biologics.
Source: Inflammatory Bowel Diseases - Category: Gastroenterology Tags: Original Article Source Type: research
Ben shared his 3-week experience living the Wheat Belly lifestyle after being diagnosed with celiac disease: “My family doctor diagnosed me with celiac and wheat allergies. These pictures were taken 3 weeks apart: prior to the diagnosis and three weeks later, a photo of me after following the Wheat Belly books that my doctor recommended I get. “I still feel like I eat like a king. I am just a lot more cautious about what I put into my body and I am seeing the results!” You can see that Ben’s face shows the changes we expect to see as inflammation recedes: reduced cheek and around-the-eyes edema, la...
Source: Wheat Belly Blog - Category: Cardiology Authors: Tags: Wheat Belly Success Stories celiac gluten grains Inflammation Source Type: blogs
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