MicroRNA ‑223 promotes hepatocellular carcinoma cell resistance to sorafenib by targeting FBW7.

MicroRNA‑223 promotes hepatocellular carcinoma cell resistance to sorafenib by targeting FBW7. Oncol Rep. 2018 Dec 06;: Authors: Tang X, Yang W, Shu Z, Shen X, Zhang W, Cen C, Cao L, Zhang M, Zheng S, Yu J Abstract Hepatocellular carcinoma (HCC) is a globally prevalent malignancy associated with a poor patient prognosis. We investigated the relationship between microRNA‑223 (miR‑223) expression and the sensitivity of HCC cells to sorafenib treatment. miR‑223 expression was determined in HCC cell lines with differential sorafenib sensitivity using reverse transcription‑quantitative PCR. miR‑223 inhibitor, miR‑223 mimic, and F‑box and WD repeat domain‑containing 7 (FBW7) short interfering RNAs (siRNAs) were transfected into the HCC cells to regulate the expression levels of miR‑223 and FBW7. Cell proliferation was evaluated using an ethynyl deoxyuridine (EdU) incorporation assay and Cell Counting Kit‑8. FBW7 protein expression levels were observed using western blotting. miR‑223 expression was increased in the HCC cells with sorafenib resistance. HCC cells with miR‑223 knockdown had significantly increased sorafenib sensitivity, but the miR‑223 mimic had the opposite effect. The TargetScan web server predicted that FBW7 is a target of miR‑223, which was confirmed by western blotting. Furthermore, FBW7 siRNA transfection increased HCC cell resistance to sorafenib in an obvious manner, and entirely eliminate...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research