Human olfactory stem cells: As a promising source of dopaminergic neuron-like cells for treatment of Parkinson's disease

Publication date: Available online 12 December 2018Source: Neuroscience LettersAuthor(s): Rafieh Alizadeh, Seyed Kamran Kamrava, Zohreh Bagher, Mohammad Farhadi, Masoumeh Falah, Fatemeh Moradi, Mahdi Eskandarian Boroujeni, Maryam Soleimani, Ahmadreza Kamyab, Ali KomeiliAbstractThe production of dopaminergic (DA) neurons from stem cells holds a great promise for future clinical treatment of neurodegenerative diseases, such as Parkinson’s disease (PD). Olfactory ecto-mesenchymal stem cells (OE-MSCs) derived from the adult human olfactory mucosa can be easily isolated and expanded in culture while maintaining their immense plasticity. In addition to reduced ethical concerns, OE-MSCs could be transplanted across allogeneic barriers, making them desirable stem cells for clinical applications. The goal of this study was to define the potentiality of human olfactory mucosa-derived MSCs aimed at differentiation into DA neuron-like cells. OE-MSCs were induced to differentiate to DA neuron-like cells in vitro by using sonic hedgehog (SHH), fibroblast growth factor 8 (FGF8), basic fibroblast growth factor (bFGF), Glial cell line-derived neurotrophic factor (GDNF) and brain derived neurotrophic factor (BDNF). Then the differentiated neurons were characterized for expression of DA neuron markers by Real-time PCR, immunocytochemistry and flow cytometry. Our findings showed that differentiated OE-MSCs could significantly express DA neuron markers at mRNA and protein levels along with dopa...
Source: Neuroscience Letters - Category: Neuroscience Source Type: research