Nudel involvement in the high glucose-induced epithelial-mesenchymal transition of tubular epithelial cells.

Nudel involvement in the high glucose-induced epithelial-mesenchymal transition of tubular epithelial cells.
Am J Physiol Renal Physiol. 2018 Dec 12;:
Authors: Zhang W, Xing LL, Xu L, Jin X, Du Y, Feng X, Liu SX, Liu Q
Abstract
Nudel is a newly discovered factor related to cell migration. The tubular epithelial-mesenchymal transition (EMT) includes four steps: the loss of the adhesive properties of epithelial cells, the acquisition of a mesenchymal cell phenotype, the destruction of the tubular basal membrane and the migration into the renal interstitium. The purpose of this study was to investigate the role of Nudel in the high glucose-induced EMT of tubular epithelial cells. Human renal proximal tubular epithelial cells (HKCs) were treated with Nudel shRNA to clarify the role and mechanism of Nudel in tubular EMT induced by high glucose. We found that Nudel was expressed at a high level in high glucose-stimulated HKCs, and the expression of Nudel was associated with the activation of STAT3. After transfection with Nudel shRNA, we detected the expression levels of E-cadherin, α-smooth muscle actin (α-SMA), and the Wiskott-Aldrich syndrome family of proteins (including WASP, N-WASP, WAVE1, WAVE2 and WAVE3) via assay. Cell migration was analyzed by the scratching method. The results showed that high glucose downregulated E-cadherin expression, upregulated α-SMA expression, and promoted the migration of HKCs. The expression levels o...

Source: American Journal of Physiology. Renal Physiology - December 12, 2018 Category: Physiology Authors: Zhang W, Xing LL, Xu L, Jin X, Du Y, Feng X, Liu SX, Liu Q Tags: Am J Physiol Renal Physiol Source Type: research