Evaluation of acute toxicity of β-lapachone associated with chitosan as a cytoprotective agent

CONCLUSION: The decrease in β -LAP toxicity after conjugation may be related to the hepatoprotective property of CS.RESUMO INTRODU ÇÃO: A β -lapachona ( β -LAP), um potente agente antitumoral, tem uso terap êutico limitado devido a sua baixa solubilidade e elevada toxicidade. Uma possível estratégia para contornar esses inconvenientes pode ser a utilização de adjuvantes como a quitosana (QS), um polissacarídeo catiônico com propriedades biológicas, como biocompatibilidade e biodegradabilidade. OBJETIVO: Avaliar a ação adjuvante da QS como citoprotetor associada à β -LAP por meio de estudos de toxicidade aguda, verificando as altera ções histopatológicas em órgãos como fígado e rins. MÉTODOS: Um conjugado da β -LAP-QS foi preparado na propor ção 1:1, administrado por via oral, com dose única da β -LAP de 80 mg/kg, em camundongos Swiss. Foram realizadas an álises histomorfológicas e histomorfométricas dos rins e do fígado desses animais. RESULTADOS: Nos estudos histomorfológicos dos grupos testados, observamos que os hepatócitos dos animais tratados com a droga livre apresentaram alterações morfológicas, como vacuolização do citoplasma, ex travasamento celular, núcleos atípicos e picnóticos. Ness...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - Category: Pathology Source Type: research

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Authors: Chen L, Luodelete M, Dong C, Li B, Zhang W, Nie P, Liu J, Chen X, Luo P Abstract Background: To investigate the pathological spectrum of glomerular disease in patients with renal insufficiency (RI) from 2008 to 2017. Methods and results: We calculated the estimated glomerular filtration rate (eGFR) with the Chronic Kidney Disease Epidemiology Collaboration creatinine (CKD-EPI) equation and defined RI as an eGFR
Source: Renal Failure - Category: Urology & Nephrology Tags: Ren Fail Source Type: research
This article describes the genetics of NS/SRNS in childhood and also gives a narrative review of the challenges and opportunities for molecular testing among children with SRNS in Nigeria. For these children to benefit from genetic diagnosis, Nigeria must aspire to have and develop the manpower and infrastructure required for medical genetics and genomic medicine, leveraging on her existing experiences in genomic medicine. Concerted efforts can be put in place to increase the number of enrollees in Nigeria's National Health Insurance Scheme (NHIS). The scope of the NHIS can be expanded to cater for the expensive bill of ge...
Source: International Journal of Nephrology and Renovascular Disease - Category: Urology & Nephrology Tags: Int J Nephrol Renovasc Dis Source Type: research
We report 4 cases of renal vasculitis or pauci-immune glomerulonephritis after checkpoint inhibitor therapy. Three patients had renal small- to medium-vessel vasculitis and 1 had focally crescentic pauci-immune glomerulonephritis. Three patients presented with acute kidney injury, and 1 presented with nephrotic syndrome and hematuria. Three patients were tested for antineutrophil cytoplasmic antibodies, which were negative. The time from checkpoint inhibitor initiation to immune-related adverse event presentation ranged from 2 weeks to 24 months. Three patients were treated with glucocorticoids, resulting in clinical resol...
Source: American Journal of Kidney Diseases - Category: Urology & Nephrology Source Type: research
We report a case of a 64-year-old man presenting with a febrile illness and acute nephritis in the setting of HGA infection. The patient’s kidney biopsy was characterized by a membranoproliferative glomerulonephritis pattern and acute interstitial inflammation. After appropriate antibiotic treatment and high-dose steroids, the patient had a marked improvement in kidney function, although a subsequent recrudescence of nephritis required a 6-month course of additional steroids. As the prevalence of tick-borne diseases continues to spread across the United States, raising awareness of the potential for atypical presenta...
Source: American Journal of Kidney Diseases - Category: Urology & Nephrology Source Type: research
Abstract Mesangioproliferative glomerulonephritis (MsPGN) is characterized by the proliferation of glomerular mesangial cells (GMCs) and accumulation of extracellular matrix (ECM), followed by glomerulosclerosis and renal failure of patients. Although our previous studies have demonstrated that sublytic C5b-9 complex formed on the GMC membrane could trigger GMC proliferation and ECM expansion of rat Thy-1 nephritis (Thy-1N) as an animal model of MsPGN, their mechanisms are still not fully elucidated. In the present studies, we found that the levels of response gene to complement 32 (RGC-32), myeloid zinc finger 1 ...
Source: J Cell Mol Med - Category: Molecular Biology Authors: Tags: J Cell Mol Med Source Type: research
Dr. Rabih Geha’s excellent post on Closler – Every Piece of Data Matters – has stimulated much thought. He makes one important point that I would like to expand. My mind finds it much harder to attach diagnostic significance to pertinent negatives. Even if their impact on a diagnostic hypothesis is equal, I find that a positive test result sways my reasoning more so than an equally impactful negative test. My students and residents know that I want to hear the numbers. Sometimes normal is not normal. Sometimes seemingly normal gives clues. The problem comes from our laboratory definition of no...
Source: DB's Medical Rants - Category: Internal Medicine Authors: Tags: Medical Rants Source Type: blogs
Publication date: Available online 8 June 2019Source: Autoimmunity ReviewsAuthor(s): Massimo Galli, Giuseppe Monti, Piero Marson, Patrizia Scaini, Maurizio Pietrogrande, Marco Candela, Laura Castelnovo, Paola Faggioli, Paola Novati, Roberta Zani, Maria Teresa Mascia, Francesco Saccardo, Cesare Mazzaro, Piercarlo Sarzi-Puttini, Marco Sebastiani, Luca Quartuccio, Salvatore De VitaAbstractObjectiveSome of the manifestations of mixed cryoglobulinemia syndrome (MCS) can be severe or life-threatening, and should be rapidly contained but, as the therapeutic approaches to such conditions are largely based on anecdotal data, a cons...
Source: Autoimmunity Reviews - Category: Allergy & Immunology Source Type: research
Publication date: June 2019Source: Journal of Microbiology, Immunology and Infection, Volume 52, Issue 3Author(s): Chung-Yi Cheng, Tso-Hsiao Chen, Yuh-Moe Sue, Wen-Sen Lee
Source: Journal of Microbiology, Immunology and Infection - Category: Microbiology Source Type: research
Authors: Monfá E, Padilla I, de León B, Vidal MJ, Barnes C, Barcía V, Martinez C, Lucas C, Sastre A, Stefan J, Prieto M PMID: 31160050 [PubMed - as supplied by publisher]
Source: Nefrologia : publicacion oficial de la Sociedad Espanola Nefrologia - Category: Urology & Nephrology Tags: Nefrologia Source Type: research
We describe a cohort of nine patients with a mean age of 57  years and known duration of HCV infection ranging 3–20 years (four with evidence of compensated cirrhosis). All patients received the ritonavir-boosted paritaprevir/ombitasvir/dasabuvir regimen for 12 weeks and achieved SVR without subsequent viral relapse. Following DAAs completion, one patie nt developed “new-onset” cryoglobulinemic glomerulonephritis, six showed either persistent or worsening glomerulonephritis, and only two patients had a complete clinical response (CCR). Of the six patients with either persistent or worsening...
Source: Clinical Rheumatology - Category: Rheumatology Source Type: research
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