Diabetes-induced neuropathic mechanical hyperalgesia depends on P2X4 receptor activation in Dorsal Root Ganglia

Publication date: Available online 8 December 2018Source: NeuroscienceAuthor(s): Juliana Maia Teixeira, Gilson Gonçalves dos Santos, Amanda Ferreira Neves, Maria Carolina Pedro Athie, Ivan José Magayewski Bonet, Catarine Massucato Nishijima, Felipe Hertzing Farias, Jozi Godoi Figueiredo, Victor Hernandez-Olmos, Samer Alshaibani, Cláudia Herrera Tambeli, Christa E. Müller, Carlos Amílcar ParadaAbstractPeripheral diabetic neuropathy (PDN) manifests in 50-60% of type I and II diabetic patients and is the major cause of limb amputation. Adequate therapy for PDN is a current challenge. There are evidences that the activation of the P2X4 receptor (P2X4R) expressed on microglial cells of the central nervous system takes part in the development of neuropathic pain. However, there is an open question: Is P2X4R activation on dorsal root ganglia (DRG) involved in the development of neuropathic pain? To answer this question, this study verified the involvement of P2X4R expressed in DRG cells on diabetes-induced neuropathic mechanical hyperalgesia in rats. We found that intrathecal or ganglionar (L5-DRG) administration of a novel P2X4R antagonist (PSB-15417) or intrathecal administration of oligodeoxynucleotides (ODN)-antisense against the P2X4R reversed diabetes-induced neuropathic mechanical hyperalgesia. The DRG of the diabetic neuropathic rats showed an increase in P2X4R expression, and the DRG immunofluorescence suggested that P2X4R is expressed mainly in satellite glial cells (...
Source: Neuroscience - Category: Neuroscience Source Type: research