The distribution and time-dependent expression of HIPK2 during the repair of contused skeletal muscle in mice.

The distribution and time-dependent expression of HIPK2 during the repair of contused skeletal muscle in mice. Histol Histopathol. 2018 Dec 05;:18072 Authors: Zhang M, Zhang MZ, Wen SH, Sun YF, Jiang PH, Wang LL, Zhao R, Wang CL, Jiang SK, Guan DW Abstract HIPK2 is an evolutionarily conserved serine/threonine kinase and is considered a co-regulator of an increasing number of transcription factors modulating a variety of cellular processes, including inflammation, proliferation and fibrosis. Skeletal muscle injuries repair is an overlapping event between inflammation and tissue repair. There are no reports about HIPK2 expression in skeletal muscles after trauma. A foundational study on distribution and time-dependent expression of HIPK2 was performed by immunohistochemical staining, Western blotting and quantitative real-time PCR, which is expected to obtain a preliminary insight into the functions of HIPK2 during the repair of contused skeletal muscle in mice. An animal model of skeletal muscle contusion was established in 50 C57B6/L male mice. Samples were taken at 1, 3, 5, 7, 9, 14, 17, 21 and 28 days after contusion, respectively (5 mice at each posttraumatic interval). 5 mice were employed as control. No HIPK2-positive staining was detected in uninjured skeletal muscle. Intensive immunoreactivties of HIPK2 were observed in polymorphonuclear cells, round-shaped mononuclear cells, regenerated multinucleated myotubes and spindle-sha...
Source: Histology and Histopathology - Category: Cytology Tags: Histol Histopathol Source Type: research