Diagnostic accuracy of molecular methods for detecting markers of antimalarial drug resistance in clinical samples of Plasmodium falciparum : protocol for an update to a systematic review and meta-analysis

The objective of this update to this systematic review is to improve and update identification of the sensitivity and specificity of each molecular method for detecting selected antimalarial drug resistance markers.MethodsWe will include diagnostic accuracy studies that compare at least two of any molecular methods to examine blood samples from patients diagnosed with, or suspected of having malaria, to detect at least one selected marker of antimalarial drug resistance. We will search PubMed, EMBASE, BIOSIS, and Web of Science from 2000 to present. Two reviewers will independently screen all results, extract data, consider applicability, and evaluate the methodological quality of included studies using QUADAS-2. We will carry out a meta-analysis and use statistical methods to compare results from homogenous studies. We will use narrative to synthesise and compare results of heterogeneous studies.DiscussionThis review will help to identify sub-optimal molecular methods for antimalarial marker detection which may be discontinued and identify more sensitive and specific methods which may be adopted. More sensitive and specific detection of drug resistance can be used to improve the breadth and accuracy of surveillance. This would enable the identification of previously undiscovered areas of antimalarial resistances and susceptibilities, improve the precision of estimates of the prevalence of resistances, and improve our ability to detect smaller changes in these patterns. Highe...
Source: Systematic Reviews - Category: International Medicine & Public Health Source Type: research

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AbstractPurpose of ReviewThe purpose of this review is to discuss the current knowledge of HIV and tissue parasite co-infection in the context of transmission enhancement, clinical characteristics, treatment, relapse, and clinical outcomes.Recent FindingsThe pathophysiology and clinical sequelae of tissue parasites in people living with HIV (PLWH) have been well described for only a handful of organisms, primarily protozoa such as malaria and leishmaniasis. Available published data indicate that the interactions between HIV and tissue parasites are highly variable depending on the infecting organism and the degree of host ...
Source: Current Infectious Disease Reports - Category: Infectious Diseases Source Type: research
ConclusionThese results indicate that A. farnesiana pods contain nematocidal compounds and might be promising natural anthelmintic agents against H. contortus. This leguminous plant could be used as a nutraceutical food source for the control of gastrointestinal nematodes in small ruminants.Graphical abstract
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
by Awtum M. Brashear, Wanlapa Roobsoong, Faiza A. Siddiqui, Wang Nguitragool, Jetsumon Sattabongkot, Margarita M. L ópez-Uribe, Jun Miao, Liwang CuiPlasmodium ovale accounts for a disproportionate number of travel-related malaria cases. This parasite is understudied since there is a reliance on clinical samples. We collected aP.ovale curtisi parasite isolate from a clinical case in western Thailand and performed RNA-seq analysis on the blood stage transcriptomes. Using bothde novo assembly and alignment-based methods, we detected the transcripts for 6628 out of 7280 annotated genes. For those lacking evidence of exp...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
(University of Bern) An international scientific consortium led by the cell biologists Volker Heussler from the University of Bern and Oliver Billker from the Ume å University in Sweden has for the first time systematically investigated the genome of the malaria parasite Plasmodium throughout its life cycle in a large-scale experiment. The researchers were able to identify hundreds of targets that are urgently needed in drug and vaccine development to eradicate the disease.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news
Malaria vector control and research rely heavily on monitoring mosquito populations for the development of resistance to public health insecticides. One standard method for determining resistance in adult mosq...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
The objective of this study was to evaluate the in vitro antiplasmodial properties against malaria parasite in 15 plants mentioned in Indian traditional medicine texts.MethodsIn vitro antiplasmodial activity of methanolic extracts obtained from Indian traditional medicinal plants was evaluated on Plasmodium falciparum of FCK2 and INDO strains using schizont maturation inhibition assay and parasite lactate dehydrogenase inhibition assay.ResultsMethanolic extracts of Adhatoda zeylanica, Embelia ribes, Piper nigrum and Plumbago zeylanica exhibited more than 50% inhibition in both the stains in schizont maturation inhibition a...
Source: Journal of Integrative Medicine - Category: Complementary Medicine Source Type: research
Parasites genes prepare it for the unexpected, but could be exploited to fight the disease More at https://www.nsf.gov/discoveries/disc_summ.jsp?cntn_id=299535&WT.mc_id=USNSF_1 This is a Research News item.
Source: NSF Discoveries - Category: Science Source Type: research
Malaria, one of the largest health burdens worldwide, is caused by Plasmodium spp. infection. Upon infection, the host ’s immune system begins to clear the parasites. However, Plasmodium species have evolved to es...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
This study describes the genetic diversity and population structure from September 2012 to March 2015 in three geographically distant settlements, Cahuide (CAH), Lupuna (LUP) and Santa Emilia (STE), located in the Peruvian Amazon. A total 777P.vivax mono-infections, out of 3264, were genotyped. Among study areas, LUP showed 19.7% of polyclonal infections, and its genetic diversity (Hexp) was 0.544. Temporal analysis showed a significant increment of polyclonal infections andHexp, and the introduction and persistence of a new parasite population since March 2013. In STE, 40.1% of infections were polyclonal, withHexp = 0.596...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Parasites genes prepare it for the unexpected, but could be exploited to fight the disease More at https://www.nsf.gov/discoveries/disc_summ.jsp?cntn_id=299535&WT.mc_id=USNSF_1 This is a Research News item.
Source: NSF Discoveries - Category: Science Source Type: research
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