GSE115524 HiChIRP reveals RNA-associated chromosome conformation

Contributors : Maxwell R Mumbach ; Jeffrey M Granja ; Ryan A Flynn ; Ansuman T Satpathy ; Adam J Rubin ; Caitlin M Roake ; Jimmy K Guo ; Yanyan Qi ; David G Gennert ; Shadi Shams ; Bryan H Louie ; Michael R Corces ; Steven E Artandi ; Paul A Khavari ; Maninjay K Atianand ; Katherine A Fitzgerald ; William J Greenleaf ; Howard Y ChangSeries Type : Genome binding/occupancy profiling by high throughput sequencing ; OtherOrganism : Homo sapiens ; Mus musculusModular domains of lncRNAs can serve as scaffolds to bring distant regions of the linear genome into spatial proximity. Here we present HiChIRP, a method leveraging novel bioorthogonal chemistry and customized 3C conditions, that enables interrogation of chromatin architecture focused around a specific RNA of interest down to ~10 copies per cell. HiChIRP of three nuclear RNAs reveal insights into promoter interactions (7SK), telomere biology (TERC), and inflammatory gene regulation (lincRNA-EPS).
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Other Homo sapiens Mus musculus Source Type: research
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