Generation of a heterozygous p53 R249S mutant human embryonic stem cell line by TALEN-mediated genome editing

Publication date: Available online 30 November 2018Source: Stem Cell ResearchAuthor(s): Zijun Huo, Jian Tu, An Xu, Li Yan, Donghui Wang, Mo Liu, Ruoji Zhou, Dandan Zhu, Yu Lin, Julian A. Gingold, Chia-Jui Yen, Haipeng Xiao, Ruiying ZhaoAbstractAs one of the most essential genome guardians, p53 and its mutants have been suggested associated with many types of cancers. Many p53 mutants function induce unique phenotypes, including carcinogenesis, metastasis, and drug resistance. The p53(R249S) mutation is the most prevalent and specific mutation associated with liver cancer development. Here, we demonstrate the generation of a heterozygous p53(R249S) mutation in the H9 human embryonic stem cell line using TALEN-mediated genome editing. The generated cell line maintains a normal karyotype, a pluripotent state and the in vivo capacity to develop a teratoma containing all three germ layer tissues.Resource tableUnique stem cell line identifierWAe009-A-11Alternative name(s) of stem cell lineH9-p53(WT/R249S) and A94–42InstitutionMcGovern Medical School, The University of Texas Health Science Center Houston, Houston, Texas, USAContact information of distributorRuiying Zhao Type of cell lineESCOriginHumanAdditional origin infoAge: N/ASex: FemaleEthnicity: N/ACell SourceH9 human Embryonic Stem CellClonalityClonalMethod of reprogrammingN/AGenetic modificationYesType of modificationInduced mutationAssociated diseaseLiver cancerGene/locus (c.747G > T)/17p13.1Method of modificationTALE...
Source: Stem Cell Research - Category: Stem Cells Source Type: research