Quantitative epigenetic analysis reveals significance of lipid metabolism regulatory miRNA-33a in defining different categories of arthritis

Publication date: Available online 30 November 2018Source: Meta GeneAuthor(s): Uroosa Ali, Chandrakala Lakireddy, Shaik Iqbal Ahmed, Syed Khaja Mujtaba Hussain, Yasmeen Owaisi, Minhaj Fatima, I.V.S.V. Raksha, Karne Divya Sree, Asma Khannam, Syed Saad Hussain, Prajakta Kale, Manisha Malini, Mazharuddin Ali Khan, Sandeep Kumar Vishwakarma, Aleem Ahmed KhanAbstractArthritis is one of the leading causes of disabilities and morbidity worldwide. Among various forms of arthritis, osteoporosis, osteoarthritis and rheumatoid arthritis have been considered the most common categories with significant pathophysiology. Although these types of arthritis torment many patients, the molecular level of pathogenesis has not been elucidated. In present study, we have identified the role of three circulating miRNAs targeting different molecular mechanisms in arthritis for identifying non-invasive diagnostic and therapeutic marker. A total of 42 arthritis patient's peripheral blood samples were collected and serum was separated. miRNA extraction was performed from serum samples and used for relative quantification of selected miRNAs (miRNA-33a, miRNA-146a and miRNA-203a) using SYBR Green-based Real-Time quantitative PCR. The results showed that all three miRNAs gets upregulated in arthritis patients however, miRNA-33a is the most significantly upregulated miRNA in total arthritis, osteoporosis, osteoarthritis and rheumatoid arthritis patient's samples as compared to other two miRNAs with respect t...
Source: Meta Gene - Category: Genetics & Stem Cells Source Type: research