In vitro and in vivo evaluation of anti-inflammatory potency of Mesua ferrea, Saraca asoca, Viscum album & Anthocephalus cadamba in murine macrophages raw 264.7 cell lines and Wistar albino rats

Publication date: Available online 29 November 2018Source: Beni-Suef University Journal of Basic and Applied SciencesAuthor(s): Syed Murthuza, B.K. ManjunathaAbstractThe present study reports the in-vitro and in-vivo anti-inflammatory potency of Mesua ferrea, Saraca asoca, Viscum album and Anthocephalus cadamba, potent medicinal plants of Western Ghats of Karnataka India. Lipopolysaccharide (LPS)-activated murine macrophage RAW 264.7 cell line was used in in-vitro assay. Pretreatment with methanolic extracts at non toxic concentrations abridged the LPS-induced protein levels. Among the four plants tested, methanolic extract of Viscum album (70.20±1.094) and Mesua ferrea (68.29±2.862) showed potent anti-inflammatory activity at 100 µg concentration with an IC50 value of 57.23±1.922 and 63.36±3.791 µg/ml. In vivo anti-inflammatory activity was evaluated using carrageenan-induced paw edema in Wistar albino rats, Viscum album (0.6167±0.01667) and Mesua ferrea (0.6833±0.01667) showed potent anti-inflammatory activity (at 100 mg/Kg.bw). The obtained results indicate the potency of active constituents of Mesua ferrea and Viscum album in the development of effective anti-inflammatory drugs.
Source: Beni Suef University Journal of Basic and Applied Sciences - Category: Science Source Type: research