Human breast cancer decellularized scaffolds promote epithelial ‐to‐mesenchymal transitions and stemness of breast cancer cells in vitro

In our study, human breast cancer biopsies were decellularized by the detergent technique with sodium lauryl ether sulfate (SLES) solution for the first time. Scaffolds seeded with MCF ‐7 cells demonstrated the process of cell recellularization in vitro, with increased cell migration, proliferation, and epithelial‐to‐mesenchymal transition (EMT) process. When treated with 5‐fluorouracil (5‐Fu), the expressions of stem cell markers, including Oct4, Sox2, and CD49F, were m aximally maintained in the recellularized scaffold with decreased apoptosis rates compared with monolayer cells. AbstractBreast cancer, with unsatisfactory survival rates, is the leading cause of cancer ‐related death in women worldwide. Recent advances in the genetic basis of breast cancer have benefitted the development of gene‐based medicines and therapies. Tissue engineering technologies, including tissue decellularizations and reconstructions, are potential therapeutic alternatives for can cer research and tissue regeneration. In our study, human breast cancer biopsies were decellularized by a detergent technique, with sodium lauryl ether sulfate (SLES) solution, for the first time. And the decellularization process was optimized to maximally maintain tissue microarchitectures and ext racellular matrix (ECM) components with minimal DNA compounds preserved. Histology analysis and DNA quantification results confirmed the decellularization effect with maximal genetic compounds removal. Quantif...
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research