Poly (3 ‐hydroxybutyrate‐co‐3‐hydroxyvalerate) improved osteogenic differentiation of the human induced pluripotent stem cells while considered as an artificial extracellular matrix

In this study, electrospun PHBV nanofibers were fabricated and characterized and then osteogenic differentiation of the human iPSCs was investigated while cultured on PHBV scaffold.‐MTT, alkaline phosphatase activity, calcium content, gene expression, and western blot evaluations were confirmed osteogenic differentiation of the human iPSCs increased significantly when grown on PHBV nanofibers. AbstractCocell polymers can be the best implants for replacing bone defects in patients. The pluripotent stem cells produced from the patient and the nanofibrous polymeric scaffold that can be completely degraded in the body and its produced monomers could be also usable are the best options for this implant. In this study, electrospun poly (3 ‐hydroxybutyrate‐co‐3‐hydroxyvalerate) (PHBV) nanofibers were fabricated and characterized and then osteogenic differentiation of the human‐induced pluripotent stem cells (iPSCs) was investigated while cultured on PHBV scaffold. MTT results showed that cultured iPSCs on PHBV proliferation were increased compared to those cultured on tissue culture polystyrene (TCPS) as the control. Alkaline phosphatase (ALP) activity and calcium content were also significantly increased in iPSCs cultured on PHBV compared to the cultured on TCPS under osteogenic medium. Gene expression evaluation demo nstrated that Runx2, collagen type I, ALP, osteonectin, and osteocalcin were upregulated in iPSCs cultured on PHBV scaffold in comparison with those cultu...
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research