Interval and Continuous Exercise Regimens Suppress Neutrophil-derived Microparticle Formation and Neutrophil-promoted Thrombin Generation under Hypoxic Stress

This study explicates the manner in which interval and continuous exercise regimens affect neutrophil-derived microparticle (NDMP) formation and neutrophil/NDMP-mediated thrombin generation (TG) under hypoxic condition. A total of 60 sedentary males were randomized to perform either aerobic interval training (AIT; 3-minute intervals at 40% and 80%VO2max) or moderate continuous training (MCT; sustained 60%VO2max) for 30 min/day, 5 days/week for 5 weeks, or to a control group who did not receive any form of training. At rest and immediately after hypoxic exercise test (HE, 100W under 12%O2 for 30 min), the NDMP characteristics and dynamic TG were measured by flow cytometry and thrombinography, respectively. Before the intervention, HE 1) elevated coagulant factor VIII/fibrinogen concentrations and shortened activated partial thromboplastin time (aPTT), 2) increased total and tissue factor-rich/phosphatidylserine-exposed NDMP counts, and 3) enhanced the peak height and rate of TG promoted by neutrophils/NDMPs. Following the 5-week intervention, AIT exhibited higher enhancement of VO2max than MCT did. Notably, both MCT and AIT attenuated the extents of HE-induced coagulant factor VIII/fibrinogen elevations and aPTT shortening. Furthermore, the two exercise regimens significantly decreased tissue factor-rich/phosphatidylserine-exposed NDMP formation and depressed neutrophil/NDMP-mediated dynamic TG at rest and following HE. Hence, we conclude that AIT is superior to MCT for enhanc...
Source: Clinical Science - Category: Biomedical Science Authors: Source Type: research