Higher Pericyte Content and Secretory Activity of Microfragmented Human Adipose Tissue Compared to Enzymatically Derived Stromal Vascular Fraction

Adipose tissue represents an abundant source of regenerative cells, easily accessible through liposuction. Lipoaspirates are used in the clinic either after enzymatic digestion into stromal vascular fraction or, as of more recently, as mechanically microfragmented fat. We now show that microfragmentation increases pericyte content, compared to plain lipoaspirate, and augments growth factor and cytokine secretion compared to stromal vascular fraction. AbstractAutologous adipose tissue is used for tissue repletion and/or regeneration as an intact lipoaspirate or as enzymatically derived stromal vascular fraction (SVF), which may be first cultured into mesenchymal stem cells (MSCs). Alternatively, transplant of autologous adipose tissue mechanically fragmented into submillimeter clusters has recently showed remarkable efficacy in diverse therapeutic indications. To document the biologic basis of the regenerative potential of microfragmented adipose tissue, we first analyzed the distribution of perivascular presumptive MSCs in adipose tissue processed with the Lipogems technology, observing a significant enrichment in pericytes, at the expense of adventitial cells, as compared to isogenic enzymatically processed lipoaspirates. The importance of MSCs as trophic and immunomodulatory cells, due to the secretion of specific factors, has been described. Therefore, we investigated protein secretion by cultured adipose tissue clusters or enzymatically derived SVF using secretome arrays....
Source: Stem Cells - Category: Stem Cells Authors: Tags: Tissue ‐Specific Progenitor and Stem Cells Source Type: research