Simultaneous analysis of acetylcarnitine, proline, hydroxyproline, citrulline, and arginine as potential plasma biomarkers to evaluate NSAIDs-induced gastric injury by liquid chromatography-tandem mass spectrometry

Publication date: Available online 24 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Soyoung Shin, Hyeon Myeong Jeong, Seung Eun Cheung, Tae Hwan Kim, Subindra Kazi Thapa, Da Young Lee, Chang Ho Song, Jun Young Lim, Sang-Min Cho, Kyu-Yeol Nam, Won-Ho Kang, Youn-Woong Choi, Beom Soo ShinABSTRACTAlthough major adverse effects associated with nonsteroidal anti-inflammatory drugs (NSAIDs) are gastric injury, assessment of NSAIDs-induced gastrointestinal adverse effects is mostly dependent on endoscopy due to the lack of plasma biomarkers. Several amino acids associated with collagenase activity and gastric mucosal mass have been suggested as plasma biomarker candidates for gastric injury. Therefore, this study aimed to develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the plasma biomarker candidates, i.e., acetylcarnitine, proline, hydroxyproline, citrulline, and arginine and evaluate their potential as a biomarker for NSAIDs-induced gastric injury. The method utilized simple protein precipitation with methanol and D4-citrulline as an internal standard (IS). The assay resulted in the lower limit of quantification (LLOQ) of 0.1 μg/mL for acetylcarnitine and 1 μg/mL for proline, hydroxyproline, citrulline, and arginine in the surrogate blank plasma. The intra- and inter-day accuracy ranged 82.5 - 111.2% for acetylcarnitine, 95.4 - 103.3% for proline, 98.9 - 106.4% for hydroxyproline, 99.5 - 103.5% fo...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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