Pseudomonas protegens MP12: A plant growth-promoting endophytic bacterium with broad-spectrum antifungal activity against grapevine phytopathogens

Publication date: Available online 23 November 2018Source: Microbiological ResearchAuthor(s): Marco Andreolli, Giacomo Zapparoli, Elisa Angelini, Gianluca Lucchetta, Silvia Lampis, Giovanni ValliniAbstractPseudomonas sp. MP12 was isolated from a soil sample collected in a typical warm-temperate deciduous forest near Brescia, northern Italy. Phylogenetic analysis identified the species as Pseudomonas protegens. We detected the genes phlD, pltB and prnC, which are required to synthesize the antifungal compounds 2,4-diacetylphloroglucinol (2,4-DAPG), pyoluteorin and pyrrolnitrin, respectively. P. protegens MP12 was also shown to produce siderophores and ammonia, yielded positive results in the indole-3-acetic acid test, and was capable of phosphate solubilization. P. protegens MP12 was able to inhibit the in vitro mycelial growth of prominent grapevine (Vitis vinifera) phytopathogens such as Botrytis cinerea, Alternaria alternata, Aspergillus niger, Penicillium expansum and Neofusicoccum parvum. The strain also showed activity against Phaeomoniella chlamydospora and Phaeoacremonium aleophilum, which cause the devastating tracheomycosis/esca disease of grapevine trunks for which there are no efficacious control methods. Moreover, MP12 strain showed in vivo antifungal activity against B. cinerea on grapevine leaves. Culture-dependent and culture-independent analysis revealed the ability of P. protegens MP12 to efficiently and permanently colonize inner grapevine tissues. These res...
Source: Microbiological Research - Category: Infectious Diseases Source Type: research