Validated analytical method to determine new salivary lipid peroxidation compounds as potential neurodegenerative biomarkers

Publication date: 5 February 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 164Author(s): Carmen Peña-Bautista, Paula Carrascosa-Marco, Camille Oger, Claire Vigor, Jean-Marie Galano, Thierry Durand, Miguel Baquero, Marina López-Nogueroles, Máximo Vento, Ana García-Blanco, Consuelo Cháfer-PericásAbstractLipid peroxidation is closely related to neurodegenerative diseases since brain shows high lipid composition and oxygen consumption. The determination of lipid peroxidation compounds in non-invasive biological samples would help to monitor the patients’ oxidative stress status. A new analytical method based on ultrasound-assisted liquid-liquid semi-microextraction (UA-LLsME) followed by Ultra Performance Liquid Chromatography coupled to tandem Mass Spectrometry was developed to determine 18 lipid peroxidation biomarkers in saliva samples. The variables affecting the UA-LLsME efficiency were systematically studied. Under the optimum conditions, the methodology was validated and showed high-throughput, high sensitivity (limits of detection 0.02-2 nmol L−1), and satisfactory precision (coefficients of variation 2–11% (intra-day) and 5–12% (inter-day)). The reliability of the described method was assessed analysing spiked saliva samples, and the recoveries were between 80% and 120% for most of the analytes. Then, the method suitability was demonstrated by analysing saliva samples (n&thin...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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ConclusionResection is the most suitable therapy for clinical symptoms of a foreign body in the upper aerodigestive tract and inflammatory complications; total thyroidectomy follows in case of malignant transformation. Thyroid heterotopy is a rare pathological condition, yet it should be taken into consideration during differential diagnosis of tumorous oropharyngeal and neck lesions.
Source: International Journal of Surgery Case Reports - Category: Surgery Source Type: research
This article discusses ultrasound in rheumatology education opportunities, competency assessment, and certification pathways.
Source: Rheumatic Disease Clinics of North America - Category: Rheumatology Authors: Source Type: research
Authors: Yoshida M, Naitoh I, Hayashi K, Jinno N, Natsume M, Hori Y, Kato A, Kachi K, Asano G, Matsuo Y, Takahashi S, Kataoka H Abstract Pancreatic serous cystic neoplasms (SCNs), such as serous cystadenoma (SCA), are generally recognized as benign because malignant counterparts of SCNs have been extremely rare. In clinical practice, pancreatic cystic neoplasms diagnosed as SCNs have been managed by conservative observation, as long as the patients remained asymptomatic. We herein report a case of metachronous ductal adenocarcinoma that was discovered during long-term follow-up of SCN and review the related literat...
Source: Internal Medicine - Category: Internal Medicine Tags: Intern Med Source Type: research
Conclusion: MFU-V is a well-known and, based on the evidence, an effective tool for non-invasive lifting and skin tightening. The secret to successful use of the device is to assess adequately patient needs and expectations and plan ahead for the combination of other treatment if necessary to achieve the desired outcome. J Drugs Dermatol. 2019;18(11):1075-1082. PMID: 31738490 [PubMed - in process]
Source: Journal of Drugs in Dermatology - Category: Dermatology Tags: J Drugs Dermatol Source Type: research
This article reviews the common MSK injuries that can be diagnosed with ultrasound with a focus on point-of-care ultrasound.
Source: Emergency Medicine Clinics of North America - Category: Emergency Medicine Authors: Source Type: research
Authors: Schilling EM, Weinrich M, Heller T, Koball S, Neumann A Abstract Our patient exhibited a large tumor on his right upper arm where his former dialysis access site had been. X-ray, Doppler ultrasound, and magnetic resonance imaging scan could not fully reveal the nature of that tumor. Eventually, a surgical approach showed a giant aneurysm of the inflowing brachial artery to a partially obliterated arteriovenous fistula. This case highlights the importance of ongoing care for patients with arteriovenous shunts. Even arteriovenous fistulas, that are obliterated or no longer in use, can, especially when immuno...
Source: The Journal of Vascular Access - Category: Surgery Tags: J Vasc Access Source Type: research
Conclusion: The classification results by CEUS and MRI are in excellent agreement. The classification of pancreatic cystic lesions by CEUS is significantly helpful for clinical diagnosis. PMID: 31737668 [PubMed - in process]
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
The objective of this work was to introduce high-intensity ultrasound (HIU) in different positions within a scraped surface heat exchanger (SSHE) and evaluate the crystallization behavior and physical properties of a low saturated, soybean-based fat. The SSHE in this study was composed of one pump (flow rate of 11 L/h), two crystallization barrels (32 °C, 344 rpm), and one pin worker (208 rpm). HIU was placed before the first crystallization step, or barrel 1 (HIU-0), between the two barrels (HIU-1), between barrels 2 and the pin worker (HIU-2) and after complete crystallization in the SSHE (HIU-3). HIU amplitu...
Source: Journal of Food Engineering - Category: Food Science Source Type: research
Publication date: Available online 18 November 2019Source: Innovative Food Science &Emerging TechnologiesAuthor(s): Oscar G. Martínez-Moreno, Luis M. Anaya-Esparza, Jorge A. Sánchez-Burgos, Libier Meza-Espinoza, Alejandro Pérez-Larios, J. Emanuel Bojorquez-Quintal, Efigenia Montalvo-GonzálezAbstractThe combined effect of vacuum, heat and ultrasound (vacuum-thermosonication, VTS) on soursop puree was investigated with regard to the viability of Escherichia coli and Staphylococcus aureus, inactivation of polyphenol oxidase and sensory quality. The VTS conditions were: vacuum (8.46, 11 and 16.9...
Source: Innovative Food Science and Emerging Technologies - Category: Food Science Source Type: research
In conventional acoustic-resolution-based photoacoustic microscopy (ARPAM), a focused ultrasound transducer is placed coaxially with the ...
Source: Applied Optics - Category: Physics Authors: Source Type: research
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