Recombinant Newcastle disease virus (NDV) La Sota expressing the hemagglutinin-neuraminidase protein of genotype VII NDV shows improved protection efficacy against NDV challenge.

Recombinant Newcastle disease virus (NDV) La Sota expressing the hemagglutinin-neuraminidase protein of genotype VII NDV shows improved protection efficacy against NDV challenge. Avian Pathol. 2018 Nov 22;:1-28 Authors: Bu YW, Yang HM, Jin JH, Zhao J, Xue J, Zhang GZ Abstract Intensive vaccination strategies against Newcastle disease (ND) have been implemented in many countries for a long time, but ND outbreaks still occur frequently, with most isolates belonging to VII genotype of Newcastle disease virus (NDV). Many researchers have revealed that vaccines closely matched to epidemic viruses provide better protection. Therefore, using a previously established reverse genetics system, we generated a recombinant NDV vaccine strain (rLa Sota-HN) based on the La Sota vaccine strain expressing the hemagglutinin-neuraminidase (HN) protein of genotype VII NDV. The pathogenicity of the recombinant virus was confirmed by the mean death time in 9-day-old specific-pathogen-free (SPF) embryonated chicken eggs and the intracerebral pathogenicity index in 1-day-old SPF chickens. Subsequently, 1-day-old chickens were immunized with commercial vaccine La Sota and recombinant virus rLa Sota-HN and then challenged with virulent genotype VII NDV strain. The results indicated that recombinant virus rLa Sota-HN provided increased protection of vaccinated chickens from morbidity and mortality, and inhibited the shedding of virulent virus after challenging...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research