Determination and Isolation of Immune Populations from Brain Tumor Microenvironments.

Determination and Isolation of Immune Populations from Brain Tumor Microenvironments. Methods Mol Biol. 2019;1884:177-188 Authors: Godino J, Schuhmacher AJ Abstract Flow cytometry analysis and fluorescence-activated cell sorting (FACS) allow the determination and isolation of different cell types from a given tumor sample. Here we describe and comment a method consisting of the preparation of a single cell suspension from a freshly dissected mouse brain tumor mass, staining with a combination of fluorescently labeled antibodies and analysis by flow cytometry to determine, characterize, and isolate different immune populations. PMID: 30465203 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research