Determination and Isolation of Immune Populations from Brain Tumor Microenvironments.
Determination and Isolation of Immune Populations from Brain Tumor Microenvironments.
Methods Mol Biol. 2019;1884:177-188
Authors: Godino J, Schuhmacher AJ
Abstract
Flow cytometry analysis and fluorescence-activated cell sorting (FACS) allow the determination and isolation of different cell types from a given tumor sample. Here we describe and comment a method consisting of the preparation of a single cell suspension from a freshly dissected mouse brain tumor mass, staining with a combination of fluorescently labeled antibodies and analysis by flow cytometry to determine, characterize, and isolate different immune populations.
PMID: 30465203 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Godino J, Schuhmacher AJ Tags: Methods Mol Biol Source Type: research