Exosome Polyphosphate Mediates the Activation of FXII By Cancer Cell-Derived Exosomes

ConclusionThese studies demonstrate that exosomes derived from both primary, non-transformed cells (HDF), as well as cancer cell lines have the ability to support FXII autoactivation in human plasma. Their ability to do is proportional to the relative risk of thrombosis associated with cancers derived from their parental cell lines. Treatment of exosomes with CIP substantially reduces the FXII activating capacity of those derived from the tested cancer cell lines and HDF. Human plasma exosomal PP is a constitutive potential source for contact activation that may be increased in cancer patients. Its relative contribution to thrombin generation via FXII activation versus that of tissue factor remains to be determined in individuals and in cancer types. However, neutralization of exosomal PP provides an additional pathway for prevention of cancer-associated thrombosis.DisclosuresSchmaier: Biomotiv: Consultancy; Alnylam: Research Funding; Enzyme Research Laboratories: Honoraria; Shire: Consultancy, Honoraria, Research Funding; Temple University: Patents & Royalties; Cleveland Clinic Foundation: Research Funding. Khorana: Pfizer: Consultancy; Sanofi: Consultancy; Janssen: Consultancy; Bayer: Consultancy.

Source: Blood - November 21, 2018 Category: Hematology Authors: You, D., Kundu, S., Schmaier, A. H., Khorana, A. A., McCrae, K. R. Tags: 331. Pathophysiology of Thrombosis: Poster III Source Type: research