Phosphatidylserine-Exposing, Tumor-Derived Microparticles Enhance Procoagulant Activity of Platelets and Endothelial Cells after Neo-Adjuvant Chemotherapy in Invasive Breast Cancer

Conclusions: We made four significant observations. (1) The PCA of patients paralleled the release of BCMPs induced by NAC. Moreover, on the second day of NAC treatment, lactadherin significantly inhibited PCA and fibrin generation, while the effect of anti-TF was minimal. (2) BCMPs induce platelet activation or even apoptosis, leading to the high PCA. (3) SEM showed that BCMPs induce the generation of fiber, impact the densities of fiber deposition and promote clot generation in a dose-dependent manner. (4) Isolated BCMPs exerted a strong cytotoxic effect on ECs, converting them to an increased procoagulant phenotype. Circulating PS+BCMPs can reflect and affect thrombogenicity and, therefore, may be used as a biomarker for hypercoagulability states.DisclosuresNo relevant conflicts of interest to declare.

Source: Blood - November 21, 2018 Category: Hematology Authors: Zhang, C., Kou, Y., Zou, L., Liang, H., Hou, L., Li, T., Zhang, Y., Li, B., Liu, Y., Zhang, J., Shi, J., WU, C. Tags: 301. Vascular Wall Biology, Endothelial Progenitor Cells, and Platelet Adhesion, Activation, and Biochemistry: Poster II Source Type: research