Kanamycin induces free radicals formation in melanocytes: An important factor for aminoglycosides ototoxicity

Previously we demonstrated that kanamycin disturbs homeostasis in light ‐pigmented melanocytes. To investigate if/how melanization contributes to this phenomenon, the study using in vitro model of dark‐pigmented melanocytes were performed. The obtained results were compared with data concerning light‐pigmented melanocytes and the role of melanin in the mechanism o f kanamycin‐induced hearing impairment was revealed. AbstractOtotoxicity is well ‐documented but not fully understood undesirable side effect of aminoglycoside antibiotic, kanamycin. Kanamycin is capable of binding to melanin biopolymers—natural pigments of the skin, hair, and eyes. Melanin‐producing cells, melanocytes, are also present in the inner ear and are known to b e necessary for normal hearing. It was considered that melanin content in the inner ear may influence aminoglycoside‐induced ototoxic effect. The impact of kanamycin on melanocytes homeostasis may thus play role in the antibiotic‐induced ototoxic effect. Previously, we demonstrated that kanamyci n disturbs homeostasis in light‐pigmented melanocytes. To investigate if/how melanization contributes to this phenomenon, the study using in vitro model of dark‐pigmented melanocytes is required. Spectrophotometric measurements and electron paramagnetic resonance (EPR) spectroscopy analysis were performed. Kanamycin induced a concentration‐dependent loss in HEMn‐DP melanocytes viability. The value of IC50 was estimated to be 5.0 ...
Source: Journal of Cellular Biochemistry - Category: Biochemistry Authors: Tags: RESEARCH ARTICLE Source Type: research