Type 1 Diabetes alters Ischemia-induced Gene Expression

Publication date: Available online 22 November 2018Source: Journal of Clinical & Translational EndocrinologyAuthor(s): Rahul Peravali, Lucas Gunnels, Satyanarayana Alleboina, Ivan C. Gerling, Ayotunde O. DokunAbstractPeripheral Artery Disease (PAD) is a chronic, activity-limiting disease that is caused by atherosclerotic occlusion of blood vessels outside the heart. Type 1 Diabetes (T1D) not only increases an individual’s likelihood of developing PAD, but also contributes to poor clinical outcomes after PAD manifestation. Although there is some evidence suggesting that hyperglycemia might alter expression of genes involved in regulating PAD severity or outcomes, our knowledge about the specific genes and pathways involved remains incomplete.We induced experimental PAD or hind limb ischemia in T1D and non-diabetic mice and subjected the ischemic gastrocnemius muscle tissues to genome-wide mRNA transcriptome and pathway analysis. We identified 513 probe sets that represented 443 different genes with highly significant expression differences (p < 0.005) between the ischemic diabetic and ischemic non-diabetic muscle tissues. Moreover, pathway analysis of the differentially expressed genes identified pathways involved in essential biological processes such as “cell cycle,” “DNA replication,” “metabolic pathways,” “focal adhesion,” “regulation of actin cytoskeleton,” and “nucleotide excision repair”. Taken together, our data offer the opportunity to te...
Source: Journal of Clinical and Translational Endocrinology - Category: Endocrinology Source Type: research