Exercise ameliorates high ‐fat diet‐induced impairment of differentiation of adipose‐derived stem cells into neuron‐like cells in rats

In this study, we determined the impact of either a 9‐week high‐fat diet (60% fat; HFD) or 9‐week exercise training on the differentiation potential of ADSCs into neuron‐like cells in male Wistar rats. Rats were randomly assigned to a normal diet‐fed (ND‐SED) group, HFD‐fed (HFD‐SED) group, or exercise‐trained HFD‐fed gro up (HFD‐EX). After a 9‐week intervention, ADSCs from all groups differentiated into neuron‐like cells. Expression of neuronal marker proteins (nestin, βIII‐tubulin, and microtubule‐associated protein 2 [MAP2]) and the average length of cell neurites were lower in cells from HFD‐SED rats than in other groups. Instead, protein expression of COX IV and Cyt‐c, the Bax/Bcl‐2 and LC3‐II/I ratio, and the malondialdehyde level in culture medium were higher in cells from HFD‐SED rats. No significant difference between ND‐SED and HFD‐EX rats was observed, except for the average length of cell neurites in MAP2. Thus, HFD impaired the differentiation potential of ADSCs into neuron‐like cells, which was accompanied by increases in apoptotic activity and oxidative stress. Importantly, exercise training ameliorated the HFD‐induced impairment of neurogenesis in ADSCs. The ad ipose tissue microenvironment could influence the differentiation potential of ADSCs, a source of autologous stem cell therapy.
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research