MicroRNA ‐1271 functions as a potential tumor suppressor in hepatitis B virus–associated hepatocellular carcinoma through the AMPK signaling pathway by binding to CCNA1

This study indicated a potential therapeutic approach of downregulation of miR ‐1271 in HBV‐associated hepatocellular carcinoma treatment. AbstractHepatocellular carcinoma (HCC) is mainly associated with hepatitis B virus (HBV) infection and characterized by metastasizing and infiltrating adjacent and distant tissues. Notably, microRNA ‐1271 (miR‐1271) is a tumor suppressor in various cancers. Therefore, we evaluate the ability of miR‐1271 to influence cell proliferation, migration, invasion, and apoptosis in HBV‐associated HCC through the Adenosine monophosphate–activated protein kinase (AMPK) signaling pathway via targ etingCCNA1. HBV ‐associated HCC and adjacent normal tissues were collected to identify the expression of miR‐1271 and CCNA1. To verify the relationship between miR‐1271 andCCNA1, we used bioinformatics prediction and the dual ‐luciferase reporter gene assay. The effects of miR‐1271 on HBV‐associated HCC cell behaviors were investigated by treatment of the miR‐1271 mimic, the miR‐1271 inhibitor, or small interfering RNA against CCNA1. The HBV‐DNA quantitative assay, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐ diphenyltetrazolium bromid assay, scratch test, transwell assay, and flow cytometry were used to detect HBV‐DNA replication, cell proliferation, invasion, migration, and apoptosis. MiR‐1271 showed a low expression, whereas CCNA1 showed a high expression in HBV‐associated HCC tissues. We identi fied that miR‐1271 ...
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research