DPPH Free-radical Scavenging and Cytotoxic Activities of Leeamacrophylla

In this study,in vitroDPPH free radical scavenging activity, total phenolic content and total flavonoid content of methanol extract from the leaves ofL. macrophyllawere determined with using UV spectrophotometric method. Antioxidant activity of the crude extract was expressed as percentage of DPPH free radical inhibition and IC50 value ( μg/ml). The IC50 value ofL. macrophylla was found to be 152.40 μg/ml. Compared to standard ascorbic acid (4.36 μg/ml),L. macrophylla leaf extract has moderate antioxidant property. The total phenolic content was 65.75 mg/g of dry weight of extract, expressed as gallic acid equivalents. The total flavonoid concentration was 59.8 mg/g, expressed as quercetin equivalents. In the brine shrimp lethality test, LC50value of crude methanolic extract obtained from the best-fit line slope was 3.06 μg/ml which was significant comparing with control (vincristine sulphate). Above results suggested that theL. macrophylla can be regarded as a promising candidate for natural plant sources of antioxidants with high value.
Source: Bangladesh Medical Research Council Bulletin - Category: International Medicine & Public Health Source Type: research