Evaluation of new biomarkers for laboratory diagnosis of tuberculosis

In this study, detection of ATP synthase subunit c (atpE) and molybdoprotein biosynthesis (moeX) genes was evaluated using loop-mediated isothermal amplification (LAMP). LAMP primers were designed by PrimerExplorer V5 online program. The atpE-LAMP could specifically detect all 28 mycobacterial reference species with no false-positive result for 14 non-mycobacterial species evaluated in this study. Only members of Mycobacterium tuberculosis complex gave a positive result by the moeX-LAMP. The analytical sensitivity of each LAMP assay was assessed using a 10-fold serial dilution of Mycobacterium tuberculosis H37Rv purified DNA. A minimum of 1 pg DNA/each reaction could be detected by both atpE- and moeX-LAMP assays. To determine the diagnostic sensitivity and specificity of the assays, 127 clinical specimens including sputum, bronchoalveolar lavge (BAL), and pleural specimens were collected during a period of one year. Dyspnea, fever, productive cough and loss of appetite were common symptoms in all patients. They were divided into two groups based of the final laboratory and clinical decisions. Fifty seven patients, who were diagnosed as having cancer, asthma, pneumonia or other non-TB diseases, were placed in non-TB group. The remaining 70 patients underwent anti-TB treatment and were considered as TB group. All the clinical specimens were subjected to DNA extraction using simple boiling method. At the time of abstract submission, the LAMP assays are conducting on the clinica...
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Tuberculosis Source Type: research