Crystallization and preliminary X-ray diffraction studies of frutalin, an α -d-galactose-specific lectin from Artocarpus incisa seeds
Frutalin is an α -d-galactose-specific carbohydrate-binding glycoprotein with antitumour properties and is a powerful tool for tumour biomarker discovery. The crystallization and preliminary X-ray diffraction analysis of this lectin, which was isolated from Artocarpus incisa seeds, are reported here. Frutalin was purified and submitted to mass-spectrometric analysis. Diverse masses at approximately 16 kDa were observed in the deconvoluted spectra, which support the presence of isoforms. The best frutalin crystals were grown within a week in 0.1 M citric acid pH 3.5 which contained 25% PEG 3350 as a precipitant at 293 K, and diffracted to a maximum resolution of 1.81 Å . The monoclinic crystals belonged to space group I2, with unit-cell parameters a = 76.17, b = 74.56, c = 118.98 Å , β = 96.56 ° . A molecular-replacement solution was obtained which indicated the presence of four monomers per asymmetric unit. Crystallographic refinement of the structure is in progress.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Monteiro-Moreira, A.C.O. D-Muniz Pereira, H. Vieira Neto, A.E. Mendes Batista Moreno, F.B. Duarte Pinto Lobo, M. Sousa, F.D. Moreira, R.A. Tags: frutalin jacalin lectin-related family Artocarpus incisa research communications Source Type: research