RNA Quality Control Using External Standard RNA.

In this study, we took into consideration the three essential factors, viz., yield of mRNA, inhibition to DNA polymerase, and degradation of mRNA for determining the RNA quality using standard RNA. It would be possible to know yield of mRNA and inhibition of the enzyme reaction by adding standard RNA before RNA extraction and looking at standard RNA loss. Degradation was evaluated by comparing the differences in the 3' and 5' regions of the RNA. In our study, it was demonstrated that in the crude extract of Saccharomyces cerevisiae , degradation was comparatively higher at the 3' end of RNA than at the 5' end. Hence, the degree of RNA degradation can be evaluated by comparing the ratio of degradation from the 3' and 5' end. PMID: 30451452 [PubMed - in process]
Source: Polish Journal of Microbiology - Category: Microbiology Tags: Pol J Microbiol Source Type: research