GSE67233 Genome-wide location of Candida albicans transcription factor Crz2p

Contributor : Sadri ZnaidiSeries Type : Genome binding/occupancy profiling by genome tiling arrayOrganism : Candida albicansCandida albicans is part of the human mycobiota, coexisting with the plethora of bacterial species within the gut. To better understand how C. albicans efficiently establishes gastrointestinal (GI)-tract colonization, we competitively challenged growth of 579 signature-tagged C. albicans strains (~ 10% genome coverage), each conditionally overexpressing a single gene, in the gut of a mouse model of GI-tract colonization. Our screen identified CRZ2, a transcription factor whose overexpression and deletion respectively increased and decreased early GI colonization. Genome-wide expression and location analyses revealed that Crz2p directly modulates the expression of many mannosyltransferase- and cell-wall protein-encoding genes, suggesting a link between Crz2p and cell-wall function. We show that CRZ2 overexpression alters glycoprotein electrophoretic mobility, confers increased sensitivity to the N-glycosylation inhibitor tunicamycin and increases C. albicans ’ adhesion to colon-tissue explants, ex vivo. Using clues from gene set enrichment analyses, we found that the optimal activity of Crz2p occurs under hypoxia at 37°C; as evidenced by both phenotypic and transcriptomic analyses following CRZ2 genetic perturbation. Our study reflects the powerful u se of gene overexpression as a complementary approach to gene deletion to identify relevant biological...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by genome tiling array Candida albicans Source Type: research