DNA polymerase β of Leishmania donovani is important for infectivity and it protects the parasite against oxidative damage.

DNA polymerase β of Leishmania donovani is important for infectivity and it protects the parasite against oxidative damage. Int J Biol Macromol. 2018 Nov 16;: Authors: Khan MI, Mishra A, Jha PK, Abhishek K, Chaba R, Das P, Sinha KK Abstract The visceral leishmaniasis is caused by L. donovani, a neglected tropical disease with an estimated number of 500,000 cases worldwide. Apart from the absence of effective vaccine, the available drugs have limitations like toxic side effects and emergence of drug resistance. The genome of Leishmania is remarkably challenged by the oxidative stress present inside the human macrophage. To maintain genomic integrity, a number of specialized DNA repair pathways assist in the recognition and repair of damaged DNA. In general, Base Excision Repair (BER) plays an essential role in the maintenance of genomic stability. We demonstrate here that the treatment of L. donovani with oxidative agents causes DNA damage and upregulation of Polβ. On the other hand, parasite overexpressing Polβ shows more resistance against Amp B, H2O2 and menadione as compared to wild type cells. We also observed a higher infectivity in the parasites that overexpress Polβ. The upregulation of Polβ was also found in stationary phase and axenic amastigote of L. donovani. Overall, we propose that Polβ is crucial for infectivity and survival of the parasite. Discovery of specific inhibitors against Polβ could offer an attr...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research

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Contributors : Kalesh Karunakaran ; Wenbin WeiSeries Type : Expression profiling by high throughput sequencingOrganism : Leishmania mexicanaWe compared the relative abundance of RNAs in the UV- cross-linked (CL) and non-cross-linked (NC) samples using total RNA-seq of both promastigotes and axenic amastigotes of L. mexicana parasites. Irradiation of the Leishmania parasites with UV-dose of 525 mJ/cm2 followed by sequential AGPC phase partitioning and proteinase-treatment of the final interface provided the CL samples for the RNA-seq analyses. The abundance of RNA species in CL and NC samples were different; ncRNAs and prot...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Leishmania mexicana Source Type: research
CONCLUSIONS: In conclusion, we disclose the conservation of the active site and interface regions, but also unique features of LbGS showing the potential of XLMS to probe structural information and explore new drugs.PMID:35019070 | DOI:10.1590/0074-02760210209
Source: Memorias do Instituto Oswaldo Cruz - Category: Infectious Diseases Authors: Source Type: research
The parasite Trypanosoma cruzi causes Chagas’ disease; both heme and ionic Fe are required for its optimal growth, differentiation, and invasion. Fe is an essential cofactor in many metabolic pathways. Fe is also harmful due to catalyzing the formation of reactive O2 species; for this reason, all living systems develop mechanisms to control the uptake, metabolism, and storage of Fe. However, there is limited information available on Fe uptake by T. cruzi. Here, we identified a putative 39-kDa Fe transporter in T. cruzi genome, TcIT, homologous to the Fe transporter in Leishmania amazonensis and Arabidopsis thaliana. ...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
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Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Short report Source Type: research
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Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
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Source: Biometals - Category: Biochemistry Authors: Source Type: research
Infect Genet Evol. 2022 Jan 3:105203. doi: 10.1016/j.meegid.2021.105203. Online ahead of print.ABSTRACTLeishmaniasis is a disease of the subtropical and tropical spheres of the earth and has various clinical manifestations. The different form of leishmaniasis includes cutaneous leishmaniasis, mucocutaneous leishmaniasis, most lethal visceral leishmaniasis and PKDL form. These different forms depend on many factors such as parasite and vector species, geographical, environmental conditions and population ethnicity. Host genetic factors have been widely investigated for their role in developing the disease in various infecti...
Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Source Type: research
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Source: Journal of Biological Inorganic Chemistry : JBIC - Category: Chemistry Authors: Source Type: research
Leishmaniasis continues to afflict known and newer endemic sites despite global efforts towards its control and elimination. In this regard, the emergence of newer endemic sites with unusual disease formats is recognized wherein Leishmania donovani complex classically known to cause visceral disease is demonstrated to cause cutaneous manifestation. In this context, atypical cutaneous leishmaniasis (CL) cases caused by L. donovani genetic variants from the newer endemic state of Himachal Pradesh (HP) in India are beginning to be understood in terms of parasite determinants. The atypical CL manifestation further needs to be ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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