Isolation and characterization of non-cellulolytic Aspergillus flavus EGYPTA5 exhibiting selective ligninolytic potential

Publication date: Available online 17 November 2018Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Mohamed S. Hasanin, Osama M. Darwesh, Ibrahim A. Matter, Houssni El-SaiedAbstractEnvironmental applications of selective lignin-degrading fungi and enzymes are of great interest as alternate technologies for paper industry (biopulping), biofuels, organic fertilizers, animal feeds. For this reason, the current study aimed to isolate selective delignifying fungi to convert the abundant lignocellulosic agricultural wastes into value-added products. From five lignocellulolytic fungi isolated from agricultural soil rich with partial decayed wooden trimmings, one isolate was selected due to its selectivity towards lignin degradation. The selected non-cellulolytic fungus isolate was identified according to morphological and molecular techniques as Aspergillus flavus EGYPTA5 with accession number MH425453. This fungal strain has been proven to cause an efficient and selective degradation of lignin in agricultural lignocellulosic wastes without affecting cellulose content. Lignin peroxidases, laccase, polyphenol oxidase, nitrate reductase and cellulase enzymes from investigating strain were assayed in the current research. All the tested enzymes except cellulase were produced with various activity degrees. Lignin peroxidases were the most active enzyme produced under experimental conditions (reached to 2.45 U/ml). Physical and chemical analysis of lignocellulosic agricultur...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research