Purification and characterization of antifungal phenazines from a fluorescent Pseudomonas strain FPO4 against medically important fungi

Publication date: September 2014 Source:Journal de Mycologie Médicale / Journal of Medical Mycology, Volume 24, Issue 3 Author(s): J.N. Gorantla , S. Nishanth Kumar , G.V. Nisha , A.S. Sumandu , C. Dileep , A. Sudaresan , M.M. Sree Kumar , R.S. Lankalapalli , B.S. Dileep Kumar The strain FPO4 was isolated from the rhizoplane of rice plant root and identified as a fluorescent Pseudomonas aeruginosa on the basis of 16S rDNA sequences and BLAST analysis. The extracellular metabolites produced by this strain were purified by silica gel column chromatography and isolated four pure compounds. Based on the spectral data the four compounds were identified as phenazin-1-ol, phenazine-1-carboxylic acid (PCA), 2-heptyl-3-hydroxyl-4(1H)-quinolone (PQS), and phenazine-1-carboxamide (PCN), respectively. Phenazin-1-ol and PCA were active against all the eight fungi tested. The highest activity of 4μg/mL by PCA was recorded against Trichophyton rubrum, a human pathogen responsible for causing athlete's foot, jock itch, ringworm and fingernail fungus infections, followed by Candida albicans and Candida tropicalis. The activity of phenazin-1-ol, PCA against Candida spp. was found to be better than the standard antifungal agent amphotericin B. Furthermore, the present study reports the antimicrobial activity of the purified phenazines on major human pathogen, T. rubrum for the first time.
Source: Journal of Medical Mycology - Category: Infectious Diseases Source Type: research