MiRNA ‐218 regulates osteoclast differentiation and inflammation response in periodontitis rats through Mmp9

AbstractPeriodontitis a multiple infection and inflammatory disease featured by connective tissue homeostasis loss, periodontal inflammation and alveolar bone resorption. MicroRNAs (miRNAs) are involved in the mediation of a large ‐scale of pathological processes. Here we show that miRNA‐218 provides protective effect on periodontitis via regulation of matrix metalloproteinase‐9 (Mmp9). This pathway is aberrant in periodontium from rats with periodontitis and HPLPCs stimulated by LPS, with downregulation of miR‐218 an d higher levels of Mmp9 compared to periodontium from healthy rats and cells without stimulation. Overexpression of miR‐218 can suppress the degradation of collagen types I, IV and Dentin Sialoprotein (DSP), attenuate osteoclast formation, and inhibit the secretion of pro‐inflammatory cytokines. On the other hand, overexpression of Mmp9 promotes the degradation of collagen types I, IV and DSP as well as RANKL‐induced osteoclast formation, and elevates inflammatory factors levels. Furthermore, the inhibitory effect of miR‐218 was prevented by rescued the Mmp9 expression. In addition, we also have showed that miR‐218 was able to attenuate bone resorption and inflammation in a periodontitis rat model. Collectively, our findings therefore suggest that miR‐218 acts as a protective role in periodontitis through the regulation of Mmp9.

Source: Cellular Microbiology - November 16, 2018 Category: Microbiology Authors: Jie Guo, Xuemin Zeng, Jie Miao, Chunpeng Liu, Fulan Wei, Dongxu Liu, Zhong Zheng, Kang Ting, Chunling Wang, Yi Liu Tags: RESEARCH ARTICLE Source Type: research