Saccharomyces cerevisiae inhibits growth and metastasis and stimulates apoptosis in HT-29 colorectal cancer cell line
AbstractColorectal cancer (CRC) is one of the leading causes of cancer-related death worldwide. Accumulating evidence has suggested that probiotics affect cellular pathways and specific genes involved in slow growth. Probiotics reduce the impact or stop the growth of cancer cells and tumors in animal models and human cell lines. The aim of this study was to investigate the effect ofSaccharomyces cerevisiae (S. cerevisiae) on cell growth, metastasis, and apoptosis of the HT-29 colon cancer cell line. The HT-29 cells andS.cerevisiae were co-cultured in order to study the effects ofS.cerevisiae on cell apoptosis, growth, and metastasis using 4′,6-diamidino-2-phenylindole (DAPI) staining, 3-(4,5-dimethylthiazoyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and scratch wound-healing assay, respectively. Real-time PCR was applied to evaluate the expression levels of Akt/PTEN, MAPK, and NF- ĸB genes. The supernatant obtained fromS.cerevisiae in HT-29 cell line increased the expression ofPTEN andCaspas3 genes in the first 24 h while theBclxl andRelA genes showed decreased expression. By using MTT method after 48 h of treatment HT-29 cells with supernatant ofS.cerevisiae, about 75% of the cells showed stopped growing. Therefore, it could be concluded thatS. cerevisiae inhibits the growth of the HT-29 cells by inducing apoptosis and reducing metastasis.
ConclusionsAn 11-point modified frailty index as measured in NSQIP correlates with readmissions after colorectal resection in patients with colon and rectal cancer.
Publication date: Available online 11 November 2019Source: Pathology - Research and PracticeAuthor(s): Can Yi, Chao Huang, Huan Wang, Chen Wang, Lijuan Dong, Xiuli Gu, Xianhong Feng, Bifeng ChenAbstractCYP24A1, an essential gene in regulation of vitamin D, has been reported to play an important role in enhancing immune activity and inhibiting tumorigenesis. Previous studies proposed that rs2585428, rs4809960, rs6022999 and rs6068816 in CYP24A1 gene might be greatly associated with cancer risk. To validate the findings, we here investigated the associations of these four polymorphisms and colorectal cancer (CRC) risk in a c...
Conclusion: An international consensus definition for the rectum is the point of the sigmoid take-off as visualized on imaging. The sigmoid take-off can be identified as the mesocolon elongates as the ventral and horizontal course of the sigmoid on axial and sagittal views respectively on cross-sectional imaging. Routine application of this landmark during multidisciplinary team discussion for all patients will enable greater consistency in tumour localisation.
ConclusionsIn this study, we constructed predictive nomograms for the presence of synchronous liver and lung metastasis in patients with colon cancer that may be used to quantitatively assess the risk of synchronous metastatic disease.
Colitis-associated cancer (CAC) is the most serious outcome of inflammatory bowel disease, which has an alteration of commensal intestinal microbiota. However, the role of intestinal microbiota on CAC progression is not well-understood. Fecal microbiota transplantation (FMT) was used for treating murine azoxymethane–dextran sodium sulfate (AOM-DSS) model of CAC. Composition of gut microbiota during FMT treatment was analyzed. RT-PCR and ELISA were used to detect the inflammatory factors, and immunofluorescence was applied to examine the phospho-nuclear factor (NF)-κB p65/p100 and Ki67-positive cells in the colo...
ConclusionsLCR may be the most useful predictive factor for OS and DFS in patients with stage II or III colon cancer.
AbstractBackgroundThe prognostic nutritional index (PNI), which is calculated using serum albumin and the peripheral lymphocyte count, is a simple and useful score for predicting the prognosis in patients with various cancers. The correlation between the preoperative PNI and long-term outcomes is unclear in oldest-old patients with colorectal cancer.MethodsA total of 84 consecutive patients ≥ 85 years old who underwent resection for primary colon adenocarcinoma at our institution between April 2008 and March 2017 were retrospectively reviewed. The cut-off value of the PNI for predicting the relapse-f...
ConclusionsExpectedly, adjuvant radiation use was relatively low but was associated with improved OS in patients with both pT4 and positive margins.
ConclusionsMRI may offer more superior diagnostic performance than CT for detecting T3-4 disease and EMVI, thereby supporting its alternative application to CT in local staging of colon cancer.