Added diagnostic value of  broad‐range 16S PCR on periprosthetic tissue and clinical specimens from other normally sterile body sites

ConclusionsOverall, the yield with 16S PCR was low. For cohort ‐1 16S PCR detected pathogens in 4.3% of culture‐negative specimens, where this was 8.2% for cohort‐2.Significance and Impact of StudyAlthough direct 16S PCR cannot replace culture, it may offer a valuable additional diagnostic option for detection of difficult to culture microorganisms in culture negative clinical specimens.This article is protected by copyright. All rights reserved.

Source: Journal of Applied Microbiology - November 15, 2018 Category: Microbiology Authors: Anne Q. Reuwer, Wouter van den Bijllaardt, Jean ‐Luc Murk, Anton G. M. Buiting, Jaco J. Verweij Tags: Original Article Source Type: research