X-ray crystallographic analysis of the catalytic domain of α -1,3-glucanase FH1 from Paenibacillus glycanilyticus overexpressed in Brevibacillus choshinensis

In this study, the recombinant catalytic domain of α -1,3-glucanase FH1 from Paenibacillus glycanilyticus FH11, which is classified into glycoside hydrolase family 87, was prepared using a Brevibacillus choshinensis expression system and purified in a soluble form. Crystals of the purified protein were produced by the sitting-drop vapor-diffusion method. Diffraction data were collected to a resolution of 1.6   Å using synchrotron radiation. The crystals obtained belonged to the tetragonal space group P41212 or P43212, with unit-cell parameters a   =   b   =   132.6, c = 76.1   Å . The space group and unit-cell parameters suggest that there is one molecule in the asymmetric unit.

Source: Acta Crystallographica Section F - November 16, 2018 Category: Biochemistry Authors: Intuy, R. Itoh, T. Suyotha, W. Hayashi, J. Yano, S. Makabe, K. Wakayama, M. Hibi, T. Tags: α -1,3-glucanase catalytic domains Paenibacillus glycanilyticus research communications Source Type: research