VARV B22R homologue as phylogenetic marker gene for Capripoxvirus classification and divergence time dating

In this study, seven genes viz. EEV maturation protein —F12L, Virion protein—D3R, RNA polymerase subunit—A5R, Virion core protein—A10L, EEV glycoprotein—A33R, VARV B22R homologue, and Kelch like protein—A55R that cover the start, middle, and end of the genome were selected. These genes were amplified from Roumanian-Fanar vaccine strain and J aipur virulent strain, cloned, and sequenced. On analysis with the available database sequences, VARV B22R homologue was identified as a marker for phylogenetic reconstruction for classifying the sheeppox viruses of the ungulates. Further, divergence time dating with VARV B22R gene accurately predic ted the sheeppox disease outbreak involving Jaipur virulent strain.
Source: Virus Genes - Category: Genetics & Stem Cells Source Type: research