Comprehensive metabolic profile of phenolic acids and flavonoids in Glechomae Herba using ultra-high-performance liquid chromatography coupled to quadrupole-time-of-flight tandem mass spectrometry with diagnostic ion filtering strategy

Publication date: Available online 16 November 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Yun Luo, Chang-Jiang-Sheng Lai, Jing Zhang, Yulin Feng, Quan Wen, Ting TanAbstractGlechomae Herba (GH), a traditional Chinese medicine, is commonly used for the treatment of urolithiasis and cholelithiasis based on its diuretic and cholagogic actions. However, relatively few studies have been conducted to describe this medicine’s metabolites. Therefore, an ultra-high-performance liquid chromatography coupled to quadrupole time of flight tandem mass spectrometry (UHPLC-QTOF-MS/MS) analysis with diagnostic product ions and neutral loss filtering strategy was established for the metabolic profiling profile of GH. First, an analytical method was established to detect the compounds in GH extracts and complex rat biosamples, including urine, bile, plasma and feces, after oral administration of GH extracts based on the holistic fingerprint approach utilizing UHPLC-QTOF-MS/MS. Second, the exogenously sourced ions were filtered out by the MarkerView software incorporated in Microsoft Office. Third, the diagnostic product ions and neutral loss filtering strategy were developed for the rapid identification of the metabolites. Finally, a total of 91 constituents, including 13 prototype compounds and 78 metabolites, were unambiguously or reasonably identified in rat biosamples. The metabolites were mainly eliminated by urine (82 metabolites) and bile (19 metabolit...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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e;pez LM, Rodríguez-Rodríguez E Abstract Cholelithiasis and kidney stones are frequent pathologies in developed countries. Gallstones can be pigmentary, cholesterol (75%) or mixed. Age, female sex, obesity, rapid weight loss, consumption of refined sugars, saturated fat, iron deficiency, vitamin D and low intake of fiber and vitamin C are factors associated with an increased risk of cholelithiasis. On the other hand, the intake of ω-3 fatty acids, oleic acid, calcium, magnesium, fiber, fruits and vegetables, dairy products, nuts, coffee, moderate consumption of alcohol, vitamin C supplements, p...
Source: Nutricion Hospitalaria - Category: Nutrition Authors: Tags: Nutr Hosp Source Type: research
Publication date: Available online 5 July 2019Source: Journal of Microbiology, Immunology and InfectionAuthor(s): Chia-Jui Yang, Hsiu-Yin Wang, Tse-Chih Chou, Chee-Jen ChangAbstractBackgroundTo determine the prevalence of chronic comorbidities and associated medication costs in Taiwanese HIV patients in order to increase awareness of the disease burden among healthcare providers and patients.MethodsHIV-diagnosed patients receiving highly active antiretroviral therapy (HAART; 2010–2013) were identified from the Taiwan National Health Insurance Research Database with the corresponding International Classification of Di...
Source: Journal of Microbiology, Immunology and Infection - Category: Microbiology Source Type: research
Publication date: Available online 3 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiaochen Liu, Xueyan Fan, Xin Wang, Ruina Liu, Caifeng Meng, Chunying WangAbstractIn traditional Chinese medicine, Lysimachiae Herba (LH) and Desmodii Styracifolii Herba (DSH) have been widely used for the treatment of calculi, but there is a certain focus in clinical application. Flavonoids as their pharmacologically active substances were focusly studied to make clear of their chemical compositions and reveal the similarities and differences between LH and DHS by analysis of characteristic marker components ...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research
Because it has become such a frequent item in everyday meals, suggesting that something so commonplace must be fine, people often ask: Is wheat really that bad? Let’s therefore catalog the health conditions that are associated with wheat consumption. Health conditions we know with 100% certainty are caused by consumption of wheat and related grains: Celiac disease, dermatitis herpetiformis, cerebellar ataxia, “idiopathic” peripheral neuropathy, temporal lobe seizures, gluten encephalopathy, type 1 diabetes, rheumatoid arthritis, autoimmune hepatitis, autoimmune pancreatitis, tooth decay Health conditions ...
Source: Wheat Belly Blog - Category: Cardiology Authors: Tags: News & Updates autoimmune diabetes gluten-free grain-free grains wheat wheat belly Source Type: blogs
Source: Drug, Healthcare and Patient Safety - Category: Drugs & Pharmacology Tags: Drug, Healthcare and Patient Safety Source Type: research
Publication date: September 2018Source: International Journal of Paleopathology, Volume 22Author(s): Emilio González-Reimers, Emilio González-Arnay, María Castañeyra-Ruiz, Matilde Arnay-de-la-RosaAbstractTiny calcified structures may be occasionally recovered during excavation of human skeletal remains. Since taphonomic processes may displace these structures from their topographical relation with neighbouring organs or bones, differential diagnoses may pose a major challenge to the archeologist and/or anthropologist. Enteroliths, kidney stones or gallstones, phleboliths, calcified ganglia, or s...
Source: International Journal of Paleopathology - Category: Pathology Source Type: research
According to the literature the three stone diseases, sialolithiasis (SL), urolithiasis (UL) and cholelithiasis (CL) share comorbidities. We assess familial and spouse risks between these stone disease and com...
Source: BMC Nephrology - Category: Urology & Nephrology Authors: Tags: Research article Source Type: research
Did you know that dehydration can severely impact your health? Staying hydrated helps your body defend itself against illnesses, prevent kidney stones, blood clots and gallstones. It is very important to stay hydrated, especially during the hot summer months. Heat will cause your body to sweat, which cools you down, but means you are losing extra fluid. When a person becomes dehydrated they typically feel very thirsty, have bouts with dry mouth or tongue furrows. They may feel exhausted and sleepy,…
Source: bizjournals.com Health Care:Physician Practices headlines - Category: American Health Authors: Source Type: news
Publication date: September 2018 Source:International Journal of Paleopathology, Volume 22 Author(s): Emilio González-Reimers, Emilio González-Arnay, María Castañeyra-Ruiz, Matilde Arnay-de-la-Rosa Tiny calcified structures may be occasionally recovered during excavation of human skeletal remains. Since taphonomic processes may displace these structures from their topographical relation with neighbouring organs or bones, differential diagnoses may pose a major challenge to the archeologist and/or anthropologist. Enteroliths, kidney stones or gallstones, phleboliths, calcified ganglia, or sesamo...
Source: International Journal of Paleopathology - Category: Pathology Source Type: research
In this study, we developed a strategy integrating targeted analysis and chemometric methods for quality evaluation and discrimination of GH from different geographical origins. Firstly, an accurate and reliable liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous quantification of fifteen phenolic constituents in GH from different geographical origins. The established method was well validated in terms of desirable specificity, linearity, precision and accuracy. Secondly, the quantitative data were subjected to the principal component analysis (PCA) and orthogonal partial least s...
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research
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