Generation of human iPSCs from human prostate cancer-associated fibroblasts IBPi002-A

Publication date: Available online 16 November 2018Source: Stem Cell ResearchAuthor(s): Zuzana Kahounová, Eva Slabáková, Lucia Binó, Ján Remšík, Radek Fedr, Jan Bouchal, Daniela Kurfűrstová, Radek Vrtěl, Vladimír Študent, Lucie Jurečková, Volodymyr Porokh, Aleš Hampl, Karel SoučekAbstractA human induced pluripotent stem cell line was generated from cancer-associated fibroblasts of a 68-years old patient with diagnosed prostate adenocarcinoma (PCa). The fibroblast cell line was reprogrammed with Epi5™ Episomal iPSC Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting expression of factors of pluripotency on a single-cell level, and also in vivo using teratoma formation assay. This new iPS cell line may be used for differentiation into different prostate-specific cell types in differentiation studies.
Source: Stem Cell Research - Category: Stem Cells Source Type: research