MicroRNA ‐99a‐5p suppresses breast cancer progression and cell‐cycle pathway through downregulating CDC25A

In this study, we aimed to explore the association between miR ‐99a‐5p andCDC25A in breast cancer and the regulatory mechanisms of miR ‐99a‐5p on breast cancer. The expressions of messenger RNA and microRNAs in breast cancer tissues and adjacent tissues were analyzed by the Cancer Genome Atlas microarray analysis. Quantitative real‐time polymerase chain reaction was conducted to find out the expression levels of miR‐99a‐ 5p andCDC25A. The expression levels of proteins (CDC25A, ki67, cyclin D1, p21, BAX, BCL ‐2, BCL‐XL, MMP2, and MMP9) were determined by Western blot analysis. The relationship between miR‐99a‐5p andCDC25A was predicted and verified by bioinformatics analysis and dual luciferase assay. After transfection, cell proliferation, invasion, and apoptosis of breast cancer tissues were, respectively, observed by cell counting kit ‐8 assay, transwell assay, and flow cytometry (FCM). Furthermore, the relationship among miR‐99a‐5p,CDC25A, and cell ‐cycle progression was determined by FCM assay. The nude mouse transplantation tumor experiment was performed to verify the influence of miR‐99a‐5p on breast cancer cell in vivo. The expression of miR‐99a‐5p in breast cancer tissues and cells was significantly downregulated, whereasCDC25A expression was upregulated. MiR ‐99a‐5p targetedCDC25A and suppressed its expression in breast cancer cells. Overexpression of miR ‐99a‐5p and decreased expression ofCDC25A could suppress breast ca...
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research