LAMTOR2/LAMTOR1 complex is required for TAX1BP1 ‐mediated xenophagy

In this study, we identified LAMTOR2 and LAMTOR1, also named p14 and p18, respectively, as previously unrecognized xenophagy regulators that modulate the autophagy receptor TAX1BP1 in response to GAS andSalmonella invasion. LAMTOR1 was localized to bacterium ‐containing endosomes, and LAMTOR2 was recruited to bacterium‐containing damaged endosomes in a LAMTOR1‐dependent manner. LAMTOR2 was dispensable for the formation of autophagosomes targeting damaged membrane debris surrounding cytosolic bacteria, but it was critical for autolysosome formation , and LAMTOR2 interacted with the autophagy receptors NBR1, TAX1BP1, and p62 and was necessary for TAX1BP1 recruitment to pathogen‐containing autophagosomes. Notably, knockout of TAX1BP1 caused a reduction in autolysosome formation and subsequent bacterial degradation. Collectively, our findings d emonstrated that the LAMTOR1/2 complex is required for recruiting TAX1BP1 to autophagosomes and thereby facilitating autolysosome formation during bacterial infection.

Source: Cellular Microbiology - November 14, 2018 Category: Microbiology Authors: Ching ‐Yu Lin, Takashi Nozawa, Atsuko Minowa‐Nozawa, Hirotaka Toh, Chihiro Aikawa, Ichiro Nakagawa Tags: RESEARCH ARTICLE Source Type: research